玉米矮花叶病毒原的生物学鉴定

THE BIOLOGICAL IDENTIFICATION OF MAIZE DWARF MOSAIC VIRUS

对我国西北的玉米矮花叶病毒B株系 (maizedwarfmosaicvirus B ,MDMV B)进行了系统的鉴定。MDMV B侵染玉米 (Zeamays)、高粱 (Sorghumvulgare)和狗尾草 (Setariaviridis)等禾本科杂草 ,但不侵染普通烟 (Nicotianatabacum )、心叶烟 (Nicotiana glutinosa)、苋色藜(Chenopodiumamaranticolor)和曼陀罗 (Daturastramonium)。MDMV B对鉴别寄主天玉 1号、熊岳 191、忻粱 7号、忻粱 52号、Atlas、Rio、Tamaran、Trudex和苏丹草 (Sorghumsudanese)的症状反应不同 ,不侵染鉴别寄主约翰逊草 (Sorghumhalepense)和燕麦 (Avenaesativa)Clintland .MDMV B呈线形 ,大小为 (72 0～ 76 0 )nm× 13nm ,提纯的MDMV B呈典型的核蛋白吸收曲线 ,MDMV B外壳蛋白亚基的相对分子质量为 36 30 0 ,是由 17种氨基酸组成的 .琼脂双扩散实验结果表明 ,MDMV B和其兔抗血清及SCMV MDB的抗血清 (美国 )都形成免疫沉淀线 .ELISA试验中 ,MDMV B和SCMV MDB的抗血清呈阳性反应 ,免疫电镜的实验结果是SCMV MDB的抗血清既能捕获MDMV B ,也能修饰MDMV B ,DIBA实验证实MDMV B与SCMV MDB的抗血清在硝酸纤维素膜上具有免疫反应 .在MDMV B侵染的玉米病叶的超薄切片中观察到病毒粒子以及风轮状 (pinwheel)和柱状 (cylindrical)内含体 .根据研究结果?

Maize dwarf mosaic virus strain B (MDMV B) from the northwest of China is identified. MDMV B can infect maize, sorghum and many grasses of Gramineae such as setaria viridis , but not Nicotiana tabacum, N. glutinosa, Chenopodium amaranticolor and Datura stramonium . There are different symptom reactions caused by MDMV B on differential hosts Tianyu 1, Xiongyue 191, Xinliang 7, Xinliang 52, Atlas, Rio, Tamaran, Trudex and Sorghum sudanese . MDMV B cannot infect differential hosts johnsongrass ( Sorghum halepense ) and Clintland. The purified MDMV B is filamentous flexuous particles about (720 760) nm×13 nm, purified virus preparation of MDMV B shows a typical ultraviolet spectrum of nucleoprotein. The molecular weight of MDMV B coat protein is 36 300 consisting of 17 kinds of amino acids. Agar gel double immunodiffusion tests show that MDMV B reacts strongly with antiserum of MDMV B and American antiserum against SCMV MDB. ELISA shows that MDMV B has close relationship with the antisera of MDMV B and SCMV MDB (U.S.A), MDMV B is serologically trapped and decorated by antiserum of SCMV MDB in immunosorbent electron microscopy test, MDMV B reacts with antiserum against SCMV MDB in Dot immunobinding assay. The pinwheel and cylindrical inclusions are observed in the maize cell infected by MDMV B. Based on the studied information, the MDMV B in the northwest of China should be SCMV MDB.