大黄对实热证模型大鼠肝基因表达谱的影响

Effects of Rhubarb on Whole Genome Expression Profiles of Liver in Fever Syndrome Model Rats

目的:利用基因芯片技术研究寒性中药大黄对2,4-二硝基苯酚致实热证模型大鼠肝脏全基因表达谱的影响,从基因水平分析大黄作用机制。方法:SPF级Wistar大鼠24只,随机分为空白对照组、模型对照组和大黄治疗组。造模后6h取肝组织,应用RatRef-12基因芯片检测各组大鼠肝脏基因表达,筛选差异表达基因,进行基因功能分类。结果:大黄水煎液组与实热模型组比较有177条差异表达基因。其中可注释基因113条,查询到29项显著性基因功能。其中生物学过程24项,主要为代谢过程功能包含59(52.21%)条差异基因;分子功能2项,主要涉及催化活性包含61(53.98%)条差异基因,其中氧化还原酶活性包含26(23%)条差异基因。结论:寒性中药大黄可调节代谢相关的基因降低机体代谢水平,实现其"清热泻火"作用。

Objective：To study the effects of Rhubarb on whole genome expression profiles of liver in fever syndrome model rats induced by 2,4-dinitrophenol based on gene chip technology,and analyze the mechanism of Rhubarb on gene level.Methods：Twenty-four Wistar rats of SPF level were randomly divided into control group,fever syndrome model group and Rhubarb group.Rats in fever syndrome model group and Rhubarb group received 2,4-dinitrophenol 2.5mg/mL by hypodermic injection,half an hour later Rhubarb group was given 10mL/kg Rhubarb decoction and the other two groups were given the same volume distilled water.After 6 hours,the livers of each group were taken out to detect the gene expression by using Illumina＇s RatRef-12 gene array.Results：There were 177 strips of gene differential expression between the Rhubarb group and the fever syndrome model group,113 of which could be annotated.Among the 113 genes,52.21% were related to the metabolism,53.98% to catalytic activity.Conclusion：Rhubarb can decrease the expression of genes related to metabolism to achieve its functions of clearing heat and purging the pathogenic fire.