HepG2^(IgG)转基因细胞应用于中药致敏物质预测研究

Prognosis of Herbal Allergy by HepG2^(IgG) Cell Line

本文对HepG2IgG细胞对于Ⅱ型致敏性预测进行了较为深入系统的研究,其中包括转染宿主细胞的筛选、转染最佳条件、最适检测仪器以及转染持续时间等。并对己知致敏原及其市售的几种中药注射剂进行了致敏性筛选。结果表明,该细胞系具有检出灵敏性高,细胞转染成功率高,致敏因子表达稳定等特点。为早期预测II型致敏反应的中药提供了一个有价值的工具。

This research was aimed to systematically study the transgenic cell line of HepG2^IgG, on the prognosis of herbal complex on allergy type Ⅱ, including screening of cells for transgenic, time for promoter transformation, instrument of detection and prolong time for transgenic passage cells. By using of these cells, we screened the known allergens and herbal injections already on the market. We demonstrate that HepG2^IgG cell has advantages of high sensitivity, success rate, and stability of IgG express for the prognosis of herbal complex on the allergy of type Ⅱ.