罗氏沼虾遗传多样性的RAPD研究

Study of RAPD on Diversity of Macrobrachium rosenbergii

采用 RAPD技术 ,对 2 0世纪 70年代从马来西亚引进的 7对罗氏沼虾后代的养殖群体 (简称 NY)和90年代从新加坡引进的天然群体的 F1与养殖群体交配繁殖的后代 (简称 NX)的遗传多样性进行了研究 .用OPM组随机引物对两个群体的基因组 DNA进行分析的结果表明 ,扩增的 DNA片段大小在 0 .1～ 1.5 kb之间 .NY群体内的平均遗传相似度 (similarity,S)为 0 .8819,NX群体内的平均遗传相似度 (S)为 0 .5 85 7.他们的遗传变异度 (variability,V)分别是 0 .1181和 0 .4143.NY群体的变异度明显小于 NX群体 .这可能与 NY群体长时期的近亲间或在小群体内繁殖导致种群退化有关 .

In order to have a sight into genetic diversity of Macrobrachium rosenbergii, Randomly Amplified Polymorphic DNA(RAPD) analysis was applied to the two populations, one(N y)of which was the progeny of the seven couples of Macrobrachium rosenbergii from Malaysian in 1 970 s,another(N x)was the filial generation of the natural population (F1) from Singapore in 1 990 s,with N y. Total 20 random primes used in this study can lead to the RAPD complication, produce amplified polymorphic DNA fragments ranging from 0.1 kb to 1.5 kb.Within N y and N x, genetic similarities(S) and variabilities(V)were 0.5857 and 0.8819(S), 0.4143 and 0.1181(V),which proves that the variation of N y are more less than of the N x. This phenomenon is to be discussed in the following article.