胚胎性横纹肌肉瘤中下调表达基因HumCyr 61的分离与克隆

solation and cloning of humCyr 61, a downstream gene in embryonic rhabdomyosarcoma

分离和克隆胚胎性横纹肌肉瘤中下调表达基因 ＨｕｍＣｙｒ ６１。方法：利用计算机辅 助的同源扩增策略，成功克隆一个长１８８７ｂｐｃＤＮＡ，并与ＤｅｎＢａｎｋ核酸数据库比较。结果：该基因 与鼠生长因子诱导早期表达的 Ｃｙｒ６１基因同源度为８２％，在蛋白质水平上的同源度高达 ９２％，其 中对高级结构有重大贡献的半胱氨酸和脯氨酸等氨基酸残基高度保守。查新结果还显示，它包括 在胚胎性横纹肌肉瘤细胞系ＲＤ中表达呈下调表达的ｃＤＮＡ片段（ＧｅｎＢａｎｋ接受号Ｚ５０１６８）和前列 腺癌患者良性组织中表达的ｃＤＮＡ片段（ＧｅｎＢａｎｋ接受号Ｙ０９８５９）。此外，该基因与鸡ＣＥＦ－１０基因 和人结缔组织生长因子（ＣＴＣＦ）基因都具有较高的同源度。结论：推测这个新基因很可能是人类的 Ｃｙｒ６１基因，故命名为 ＨｕｍＣｙｒ ６１（ＧｅｎＢａｎｋ注册号 ＡＦ０３１３８５）。

Aim: To isolate and clone humCyr 61, a downstream gene in embryonic rhabdomyosarco- ma. Methods: With amplification strategy addicted by computer, a novel 1887bp cDNA was successfully cloned and compared with the sequences in GeneBank. Results: the homology between the gene and Gyr 61 (A gene could be induced to express by mouse growth factor) was 82% in DNA sequence, and 92% in protein sequence. among them, Cystein residue and Proline residue highly conserved. This gene was included in cDNA fragments of embryonic rhabdomyosarcoma cell line RD(CenBank Reception Number: Z50168) and benign tissue of prostatic cancer (GenBank Reception Number: Y09859). In addition, there was homology between the gene and chicke CEF - 10 gene or human connective tissue growth factor (CTGF) gene. Conclusion: It was speculated that it might be human Cyr 61 gene and named HumCyr 61 (GenBank Register Number: AF 031385).