甘氨酸对内毒素诱导单个核细胞产生IL-1和TNF的影响

Inhibitory effect of glycine on the production of monocytic IL-1 and TNF induced by ET

研究甘氨酸（ｇｌｙｃｉｎｅ，Ｇｌｙ）对内毒素（Ｅｎｄｏｔｏｘｉｎ，ＥＴ）诱导单个核细胞产生ＩＬ－１和ＴＮＦ的影响。方法：用促进小鼠胸腺细胞增殖反应法测定ＩＬ－１的含量，用ＥＬＩＳＡ法测定ＴＮＦ含量。结果：ＥＴ组与ＥＦ＋Ｇｌｙ组７个样品浓度的单个核细胞培养上清液中ＩＬ－１放免活性（ＣＰＭ）值，通过配对资料单因素方差分析差异有非常显著的统计学意义（ Ｐ＜ ０． ０１）；ＥＴ组ＴＮＦ含量与ＥＴ＋Ｇｌｙ组ＴＮＦ含量比较也有非常显著性差异（Ｐ＜０．０１），而单纯Ｇｌｙ组与ＮＳ对照组比较无显著性差异（Ｐ＞０．０５）。结论：表明Ｇｌｙ能抑制ＥＴ诱导ＩＬ－１的产生；说明ＥＴ＋Ｇｌｙ组ＴＮＦ的产生被明显抑制，而单纯 Ｇｌｙ不能诱导 ＴＮＦ的产生。

Aim: The inhibitory effect of Gly on monocytic IL - 1 production induced by ET was observed. Methods: Using murine - thmocytes - proliferation method to test the IL - 1 activity, and ELISA method to test the TNF content. Samples were diluted to 7 concentrations. Results: IL - 1 contents in ET - incubated supernants and ET + GLY - incubated supernatants existed clear difference between two groups(P < 0.01 ). There was definite difference in TNF content between ET and ET + GLY group(P < 0. 01 ), while no clear difference between GIY and NS group(P > 0 .05 ). Conclusion: These results indicated that Gly could antagonize IL - 1 production induced by ET. TNF production in ET + GLY group was definitely inhibited.