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蛋氨酸脑啡肽对SH-SY5Y细胞的生长抑制作用及其对细胞内钙离子浓度的影响 被引量:6

Effects of methionine enkephalin on the growth inhibition and intracellular calcium concentration of SH-SY5Y cells
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摘要 目的研究蛋氨酸脑啡肽抗肿瘤作用及对SH-SY5Y细胞内钙离子浓度影响。方法用CCK-8法,检测蛋氨酸脑啡肽对SH-SY5Y细胞的生长抑制作用;显微镜下记录给药前后细胞生长情况;流式细胞术结合Fluo 4-AM染色法,检测细胞内钙离子浓度变化,用SAS 9.1统计分析。结果蛋氨酸脑啡肽可通过降低细胞内钙离子浓度对SH-SY5Y细胞生长产生浓度特异性抑制作用。结论细胞内钙离子浓度变化参与蛋氨酸脑啡肽抑制SH-SY5Y肿瘤细胞的生长。 Objective To investigate the anti-tumor effect and influence of calcium ion concentration in SH-SY5Y cells of methionine enkephalin.Methods The growth inhibition of SH-SY5Y cells with methionine enkephalin was detected by CCK-8 assay.The cell growth of SH-SY5Y was recorded by the inverted electron microscopy before and after administration.The intracellular concentration of calcium ion before and after administration were detectd by flow cytometry and Fluo 4-AM staining.Using SAS 9.1 software for tatistical analysis of data.Results Methionine enkephalin has obvious inhibition effect of SH-SY5Y cell growth,and there is an optimal inhibitory concentration.The drug does not kill the cell,and it can reduce the intracellular concentration of calcium,regulating the activity of cell signaling systems.Conclusion The concentration of intracellular calcium ion involved in the tumor cells SH-SY5Y growth inhibition by methionine enkephalin.
出处 《中国临床药理学杂志》 CAS CSCD 北大核心 2012年第11期834-836,共3页 The Chinese Journal of Clinical Pharmacology
关键词 蛋氨酸脑啡肽 细胞生长抑制 钙离子浓度 methionine enkephalin cell growth inhibition calcium ion concentration
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  • 1Zagon IS, Hytrek SD, Smith JP, et al. Opioid growth factor(OGF) inhibits human pancreatic cancer transplanted into nude mice [ J ]. Cancer Lett,1997 ;112:167 - 175.
  • 2Cheng F, McLaughlin PJ, Verderame MF, et al. The OGF - OGFr axis utilizes the pl6INK4a and p21WAF1/CIP1 pathways to restrict normal cell proliferation[ J]. Mol Biol Cell, 2009 ; 1:319 - 327.
  • 3Liu XH,Huang DA, Yang FY, et al . A new cytokine : the possible effect of pathway of methionine enkephalin [ J ]. World J Gastroentero1,2003 ;9 : 169 - 173.
  • 4药立波,冯作化,周春燕.医学分子生物学(第3版)[M].北京人民卫生出版社,2008:8,107.

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  • 1Castellanos A, Vicente-Dueas C, Campos-Shnchez E, et al. Cancer as a reprogramming-like disease: implications in tumor development and treatment[J]. Semin Cancer Biol, 2010, 20(2): 93-97.
  • 2McLaughlin PJ, Zagon IS. The opioid growth factor-opioid growth factor receptor axis: homeostatic regulator of cell proliferation and its implications for health and disease[J]. Biochem Pharmacol, 2012, 84(6): 746-755.
  • 3Liu XH, Huang DA, Yang FY, et al. A new eytokine: the possible effect of pathway of methionine enkephalin[J]. World J Gastroenterol, 2003, 9(1): 169-173.
  • 4Zagon IS, McLaughlin PJ. Targeting opioidergic pathways as a novel biological treatment for advanced pancreatic cancer[J]. Expert Rev Gastroenterol Hepatol, 2012, 6(2): 133-135.
  • 5Rodriguez-Milla MA, Mirones I, Mari as-Pardo L, et al. Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients[J]. Int J Mol Med, 2012, 30(2): 365-373.
  • 6Cheng F, McLaughlin P J, Verderame MF, et al. Dependence on nuclear localization signals of the opioid growth factor receptor in the regulation of cell proliferation[J]. Exp Biol Med (Maywood), 2009, 234(5): 532-541.
  • 7Cheng F, McLaughlin P J, Verderame MF, et al. The OGF-OGFr axis utilizes the P16INK4a and P21wArl/cIPl pathways to restrict normal cell proliferation[J]. Mol Biol Cell, 2009, 20(1): 319-327.
  • 8Kamb A. Cell-cycle regulators and cancer[J]. Trends Genet, 1995, 11(4):136-140.
  • 9Lee DH, Kim C, Zhang L, et al. Role of P53, PUMA, and Bax in wogonin-induced apoptosis in human cancer cells[J]. Biochem Pharmacol, 2008, 75(10): 2020-2033.
  • 10Lam M, Carmichael AR, Griffiths HR. An Aqueous extract of Fagonia cretica induces DNA damage, cell cycle arrest and apoptosis in breast cancer cells via FOXO3a and P53 expression[J]. PLoS One, 2012, 7(6): e40152.

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