摘要
目的研究蛋氨酸脑啡肽抗肿瘤作用及对SH-SY5Y细胞内钙离子浓度影响。方法用CCK-8法,检测蛋氨酸脑啡肽对SH-SY5Y细胞的生长抑制作用;显微镜下记录给药前后细胞生长情况;流式细胞术结合Fluo 4-AM染色法,检测细胞内钙离子浓度变化,用SAS 9.1统计分析。结果蛋氨酸脑啡肽可通过降低细胞内钙离子浓度对SH-SY5Y细胞生长产生浓度特异性抑制作用。结论细胞内钙离子浓度变化参与蛋氨酸脑啡肽抑制SH-SY5Y肿瘤细胞的生长。
Objective To investigate the anti-tumor effect and influence of calcium ion concentration in SH-SY5Y cells of methionine enkephalin.Methods The growth inhibition of SH-SY5Y cells with methionine enkephalin was detected by CCK-8 assay.The cell growth of SH-SY5Y was recorded by the inverted electron microscopy before and after administration.The intracellular concentration of calcium ion before and after administration were detectd by flow cytometry and Fluo 4-AM staining.Using SAS 9.1 software for tatistical analysis of data.Results Methionine enkephalin has obvious inhibition effect of SH-SY5Y cell growth,and there is an optimal inhibitory concentration.The drug does not kill the cell,and it can reduce the intracellular concentration of calcium,regulating the activity of cell signaling systems.Conclusion The concentration of intracellular calcium ion involved in the tumor cells SH-SY5Y growth inhibition by methionine enkephalin.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2012年第11期834-836,共3页
The Chinese Journal of Clinical Pharmacology
关键词
蛋氨酸脑啡肽
细胞生长抑制
钙离子浓度
methionine enkephalin
cell growth inhibition
calcium ion concentration