不同剂量奥沙利铂对小鼠皮下移植肿瘤的抑制效果

Inhibition effect of different doses oxaliplatin on the growth of tumor transplanted froma mouse

目的观察不同剂量奥沙利铂对小鼠皮下移植肿瘤的抑制效果。方法建立胃癌小鼠模型,随机分3组:5%葡萄糖20 mL.kg-1对照组,小、大剂量(1.5,15.0 mg.kg-1)奥沙利铂组,腹腔等容积注射给药,连续10 d,第11 d处死小鼠,计算瘤重抑制率、肿瘤微血管密度,检测caspase-3活性,用Westernblot法检测VEGF和Ang-2的表达。结果小、大剂量(1.5,15.0 mg.kg-1)奥沙利铂组的抑瘤率分别为44.7%和15.8%(P<0.05)。2个剂量奥沙利铂组的caspase-3活性较对照组明显增加;但是小剂量奥沙利铂的caspase-3活性高于大剂量组(P<0.05)。小剂量奥沙利铂的的微血管密度、VEGF和Ang-2的表达较对照组明显下降(P<0.05)。结论小剂量奥沙利铂抑瘤作用明显,并具有抗肿瘤血管新生的作用。

Objective To observe the inhibition effect of different doses oxaliplatin on the growth of tumor transplanted froma mouse.Methods Amouse tumor model was set up by the SGC-7901 cell line and divided into three groups： including control 5% glucose injection group,oxaliplatin 1.5 mg·kg-1 group and oxalip latin 15.0 mg·kg-1 group.Since the second day,the three groups were injected introperitoneally with same amount,once a day for 10 days.Oxaliplatin 15.0 mg·kg-1 conventional chemotherapy group was injected introperitoneally on the second day.After 10 days,the mice were killed and the tumors were taken out and the tumor weight inhibiting rates calculated.Tumors were examined by using immunohistochemical staining to study the expression of CD34.The activity of caspase-3 was measured and the expression of VEGF and Ang-2 were detected.Results The tumor weight inhibiting rates in oxaliplatin1.5 mg·kg-1 group and oxaliplatin 15.0 mg·kg-1 group were 44.7% and 15.8%.The activity of caspase-3 was increased in oxaliplatin1.5 mg·kg-1 group and oxaliplatin 15.0 mg·kg-1 group,but the activity of caspase-3 was much higher in oxaliplatin 1.5 mg·kg-1 group（P0.05）.Compared with negative control group,MVD and the expression of VEGF and Ang-2 in small dose oxaliplatin group were much lower（P0.05）.Conclusion Low dose of oxaliplatin shows significant inhibition effect on the growth of tumor and the antiangiogensis effect.