摘要
为有效治理石油污染土壤,从长期遭受石油污染的土壤中筛选出一株烃降解菌8-11作为出发菌,利用低能N+注入烃降解菌进行诱变,在能量为20 keV、剂量为90×2.6×1013ions/cm2条件下筛选出一株高效烃降解菌——诱变菌23。原油摇瓶发酵实验表明诱变菌对原油的降解率达到74%;降解后原油的全烃气相色谱图显示,经过7 d的作用,原油中的正构烷烃完全降解。诱变菌23能够产生大量的生物表面活性物质,傅里叶红外光谱分析表明其产生的生物活性物质为糖脂类化合物,该糖脂类生物表面活性剂能使发酵液的表面张力从空白对照的56.1 mN/m降低为29.3 mN/m。研究表明诱变菌23具有较高的烃降解能力,能有效降低表面张力,具有较大的应用潜力。
To remediate petroleum-contaminated soil effectively,bacteria 8-11,isolated from oil contaminated soil in Daqing Oilfield,was implanted with low energy N＋（90×2.6×1013 ions/cm2 and 20 keV）,and finally the strain of mutant 23 which could efficiently degrade oil was obtained.The degradation of petroleum hydrocarbon by mutant 23 was investigated,and results indicated that the biodegradation rate of total petroleum hydrocarbon（TPH） reached 74% after 7d treatment,and n-alkane of oil composition was degraded completely.Biosurfactants,which produced by mutant 23 and mainly indentified as glycolipid by FT-IR analysis,could reduce the surface tension of culture from 56.1 mN/m（CK） to 29.3 mN/m.Characteristics of bacterial mutant 23 suggested its potential application in bioremediation of petroleum-contaminated soil.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2012年第11期41-45,共5页
Environmental Science & Technology
基金
国家自然基金(10905035)
湖北省教育厅优秀中青年项目(Q20081201)
关键词
烃降解菌
生物修复
生物表面活性剂
离子注入
气相色谱
石油污染土壤
hydrocarbon-degrading bacteria
bioremediation
biosurfactant
ion implantation
gas chromatography
petroleum-contaminated soil
