猪肝微粒体体外代谢2,2’,4,4’-四溴联苯醚

In Vitro Metabolism of 2,2',4,4'-Tetrabromo Diphenyl Ether by Pig Liver Microsomes

多溴联苯醚(PBDEs)是一类在环境中普遍存在的持久性有机污染物,研究PBDEs的体外代谢行为对理解其在体内的富集和转化具有重要意义。文章以猪肝微粒体作为研究对象,以体外代谢形式研究其对2,2’,4,4’-四溴联苯醚(BDE-47)的代谢能力,优化了代谢条件,并研究了0.01、0.05、0.1、0.5、1、5、10 mg/L的BDE-47对细胞色素P450酶系中7-乙氧基香豆素-O-脱乙基酶(ECOD)、7-乙氧基异吩唑酮-O-脱乙基酶(EROD)和苯胺4-羟基化酶(ANH)活性的影响。结果表明,孵育0.1 mg/L的BDE-47时,猪肝微粒体对BDE-47的代谢率可达到28.6%。实验所设各浓度BDE-47均能够保护微粒体的ECOD活性,但较高浓度的BDE-47(0.5、1、5 mg/L)对微粒体的EROD活性有显著抑制作用,而各浓度BDE-47对ANH活性没有显著影响。

Polybrominated diphenyl ethers（PBDEs）are a kind of persistent organic pollutants,which are ubiquitous in environment.It is of great significance to investigate metabolic behavior of PBDEs in vitro for a better understanding of their enrichment and transformation in vivo.Metabolic ability of pig liver microsomes to 2,2＇,4,4＇-tetrabromo diphenyl ether（BDE-47）in vitro was studied.Optimal metabolic conditions and influences of BDE-47 at 0.01,0.05,0.1,0.5,1,5 and 10 mg/L on activities of 7-ethoxycoumarin O-deethylase（ECOD）,7-ethoxyresorufin O-deethylase（EROD） and aniline p-hydroxylase（ANH） of cytochrome P450 enzymes were investigated.Results showed that BDE-47 could be transformed by pig liver microsomes when the exposure concentration was 0.1 mg/L,and metabolic rate was about 28.6%.BDE-47 at various concentrations set could protect the ECOD activity of microsomes,while BDE-47 at the concentration of 0.5,1 and 5 mg/L could inhibit the EROD activity significantly,and no significant effects were observed on ANH activity.