Fetuin-a对3T3-L1脂肪细胞增殖和脂解的影响

Effect of Fetuin-a on Proliferation and Lipolysis of 3T3-L1 Adipocytes

目的观察胎球蛋白-a(fetuin-a)对体外培养的3T3-L1脂肪细胞增殖和脂解的影响。方法体外培养小鼠3T3-L1前脂肪细胞,以MTT法检测3T3-L1前脂肪细胞的增殖状况;采用甘油检测试剂盒测定释放到上清液的甘油含量作为脂解率的指标;采用Western blotting检测细胞内磷酸化激素敏感脂肪酶(hormone sensitive lipase,HSL)和脂肪甘油三酯脂肪酶(adipose triglyceride lipase,ATGL)的蛋白表达。结果不同浓度的fetuin-a在干预3T3-L1前脂肪细胞后明显促进细胞增殖,且呈剂量依赖性(P<0.05)。Fetuin-a能够抑制成熟脂肪细胞的脂肪分解,降低磷酸化的HSL及ATGL蛋白表达,且呈剂量依赖性(P<0.05)。结论 Fetuin-a通过促进3T3-L1前脂肪细胞增殖及抑制成熟脂肪细胞的脂解参与肥胖的发生。

Objective To observe the effect of fetuin - a on proliferation and lipolysis of 3T3 - L1 adipocytes. Methods 3T3 - L1 preadipocytes were cultured and induced in vitro. The proliferation of 3T3 - L1 preadipocytes was detected by MTT method. Lipolysis of adipocytes was examined by the measurement of glycerol release. The expressions of protein of phospho - HSL,ATGL were analyzed using western blot. Results The proliferation of 3T3 - L1 preadipocytes was stimulated significantly by fetuin - a （ P 〈 0.05）. Fetuin - a in- hibited lipolysis in adipocytes in a dose - dependent manner（ P 〈 0.05 ）. Fetuin - a decreased the expressions of phospho - HSL and AT- GL protein（P 〈 0.05）. Conclusion Our study provides the evidence that fetuin - a might participate in obesity via its influence on the proliferation and lipolysis of adipocytes.