摘要
目的体外研究磷酸二酯酶4抑制剂Hemay005对人白细胞趋化活性的影响。方法体外分离和培养人外周血白细胞,用甲酰三肽(fMLP)作诱导剂,通过48孔微量趋化小室检测Hemay005人白细胞趋化功能的影响。结果 Hemay005与同类PDE4抑制剂CC-10004对fMLP诱导的人白细胞迁移均有明显抑制,抑制作用呈剂量依赖性,IC50值分别为0.188μg/ml和0.253μg/ml。结论 Hemay005可通过抑制炎性反应的中心环节-白细胞趋化而发挥抗银屑病作用。
Objective To evaluate the effects of the phosphodiesterase 4 inhibitor Hemay005 on human leukocytes chemotaxis in vitro. Methods Human peripheral leucocytes were isolated and cultured in vitro. The effects of Hemay005 on leukocytes chemotaxis in- duced by f - Met - Leu - Phe （fMLP） were detected with 48 - well chemotactic chamber assay. Results Both of Hemay005 and CC - 10004 can significantly inhibit leucocytes chemotactic activities towards fMLP and the inhibition effects were shown in a dose - dependent pattern. The 50% inhibitory concentration value （IC50） was 0. 188 and 0. 253μg/ml, respectively. Conclusion Hemay005 can play the role of antipsoriasis by inhibiting leukocytes chemotaxis which is the key step of inflammatory response.
出处
《医学研究杂志》
2012年第11期44-47,共4页
Journal of Medical Research
关键词
白细胞趋化
炎症
银屑病
磷酸二酯酶4抑制剂
Leukocytes chemotaxis
Inflammation
Psoriasis
Phosphodiesterase 4 inhibitors
