尾静脉高压注射hIL-10质粒对去卵巢大鼠实验性牙周炎发生的影响

Effect of The hIL-10 Gene Transfered by Hydrodynamic Tail Vein Injection on Experimental Periodontitis in Ovariectomized Rats

目的探讨hIL-10基因治疗对去卵巢大鼠骨代谢、牙槽骨的影响。方法将96只SD大鼠随机分成4组(SHAM+hIL-10组,SHAM+VECTOR,OVX+hIL-10组,OVX+VECTOR组),每组24只。术后即刻将各组大鼠随机分成3小组,分别于术后12、14、18周建立实验性牙周炎模型。术后12周起,每周给予尾静脉加压注射hIL-10质粒(1、3两组)或空载体质粒(2、4两组)1次。丝线结扎后2周,处死大鼠。结果 hIL-10基因可在血清中表达,抑制血清TNF-α和TRAP 5b的升高(P<0.05)。大鼠牙周膜的IL-1β和TNF-α阳性细胞数目不同程度地减少(P<0.05)。结论 hIL-10基因治疗不能抑制去卵巢大鼠实验性牙周炎的牙槽骨吸收,但对牙周膜IL-1β和TNF-α有抑制作用。

Objective To investigate the effect of the hIL - 10 gene transfered by hydrodynamic tail vein injection on bone metabo- lism, absorption of alveolar bones in ovariectomized rats. Methods Ninety - six SD female rats were randomly divided into 4 equal groups：group Ⅰ （ SHAM ＋ hIL - 10） ,group Ⅱ （ SHAM ＋ Control vector （VECTOR） ,group Ⅲ （ ovx ＋ hIL - 10） ,group Ⅳ（ OVX ＋ VEC- TOR）. Twelve weeks after surgery, the rats were transferred with hIL - 10 or vector plasmids by hydrodynamic tail vein injection weekly. Seven days after the second, fourth and eighth injection, 8 rats were sacrificed from each group. Two weeks before being sacrificed, the left upper second molars were assigned to receive silk ligatures to induced experimental periodontitis. Results It was demonstrated that the expressed hIL - 10 protein in serum after gene transfer downregulated the serum expression levels of TNF - sand TRAP 5b in ovariec- tomized rats （ P 〈 0.05 ）. The numbers of IL - 1 βand TNF - αpositive cells in the furcation decreased significantly （ P 〈 0.05 ）. Conclu- sion Although the numbers of IL - 1β and TNF - α positive cells in the furcation of ovariectomized rats were decreased, hIL - 10 gene transferred by hydrodynamic tail vein injection had no significant effect on the absorption of alveolar bone.