无症状携带献血者HTLV-Ⅰ病毒的前病毒长末端核酸序列分析

Nucleotide Sequencing of Long Terminal Repeat Gene of HTLV-Ⅰ Provirus on a Multiple Asymptomatic Donor

目的了解本地区检出的无症状献血人群携带人类嗜T淋巴细胞病毒(HTLV)株亚型。方法对3份HTLV-Ⅰ/Ⅱ血清阳性标本,采用Nested-PCR方法检测前病毒长末端重复序列(1ong terminal repeat,LTR)并测序分析。结果从1名有多次合格献血经历,但抗体筛查强阳性(OD=3.000)血样中提取到HTLV-Ⅰ前病毒DNA。核苷酸序列分析提示,与日本原型ATK株比较其序列一致性为98.4%(738/750)。结论试验认定该HTLV病毒为世界群b型日本型毒株,并提示无症状携带HTLV-Ⅰ病毒的献血者具有潜在经血传播的风险。

Objective To understand the HTLV virus subtypes and origins detected from our local asymptomatic blood population. Methods Three seropositive samples of HTLV -Ⅰ /Ⅱ antibodies were subsequently tested by nested polymerase chain reaction （PCR） for long terminal repeat gene （LTR） of HTLV -Ⅰ provirus and then sequenced. Results The HTLV -Ⅰ provirus DNA was obtained from one eligible blood donor with the records of six consecutive donations, but strongly positive（ OD = 3. 000） in antibody screening tests. The identity of nudeotlde sequencing was 98.4% （738/750） compared with Japanese ATK strain. Conclusion Our test confirmed the HTLV virus to be the world＇s group of b - type and indicated that there presented a potential risk to be transmitted through transfusion by asymptomatic donors.