摘要
对日本东海地区无形体病原菌(A.phagocytophilum)的媒介蜱虫(金沟硬蜱和卵形硬蜱)的唾液腺DNA进行提取,应用分子生物学技术在亚洲地区成功地解读了p44主要外膜蛋白2.87kb的基因序列,并与欧美地区A.phagocytophilum病原体的p44主要外膜蛋白基因进行序列比对,确定了p44主要外膜蛋白表达基因在欧美以及亚洲地域间的差异性.
In this study,the salivary gland DNA extraction of ticks,a carriers of A.phagocytophilum inhabiting in eastern ocean area of Japan was investigated by variety of molecular biological technology,and for the first time a 2.87 kb of p44 outer membrane protein encoding gene sequences in Asian area was successfully characterized.Comparative analysis was performed with the strains isolated from US and European area.The finding of this study could form a base for further comprehensive study of A.phagocytophilum and the prevention of Human anaplasmosis diseases.
出处
《内蒙古师范大学学报（自然科学汉文版）》
CAS
北大核心
2012年第5期518-523,530,共7页
Journal of Inner Mongolia Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(201081072894)
