中国西门塔尔牛DGAT1和DGAT2基因组织表达谱及其在脂肪组织中的表达与背膘厚的关联分析

The Tissues Profile of DGAT1 and DGAT2 and the Association Analysis of Their Expression in the Adipose with Backfat Thicknessin Chinese Simmental Cattle

为探讨二脂酰甘油酰基转移酶1和2基因(DGAT1和DGAT2)发育性表达特点及其与背膘厚的关系,采用137头肉用中国西门塔尔牛,屠宰并采集组织样品,根据胴体背膘厚度分成高、低2组,每组各选择15个个体,利用荧光定量PCR技术,对DGAT1和DGAT2基因在组织中的表达特征及其在不同个体背膘组织中的表达水平进行分析。研究发现,在睾丸、肾脏、肋间肉、心脏、胰脏、背膘、肩肉、肝脏、网脂、肠脂、脾脏、肺、眼肉和淋巴等多种组织中均有不同程度的表达,DGAT1和DGAT2在不同组之间的表达差异显著(P<0.05),且与背膘厚度呈显著相关(P<0.05)。结果表明,DGAT1和DGAT2与肉牛背膘厚度显著相关(P<0.05),是影响肉牛肥育性状的候选基因。

The present study discussed the developmental expression characteristics of the acyl CoA diacylglycerol acyltransferase genes 1 and 2（DGAT1 and DGAT2） and their relationship to backfat thickness（BF）.30 samples from 137 slaughtered Chinese Simmental cattle were divided into thick（H） and thin（L） backfat groups,and the DGAT1 and DGAT2 genes′ expression characteristics and its expression levels in different individuals adipose tissue were analyzed by quantitative PCR technique.The result indicated that both the DGAT1 and DGAT2 had different expressed in the testis,kidney,intercostal muscle,heart,pancreas,backfat,shoulder muscle,liver,greater omental fat,gut fat,spleen,lung,rib eye muscle and lymph.The DGAT1 and DGAT2 mRNA in adipose were higher in H group than those in L group（P0.05）,and correlated with backfat thickness（P0.05）.The results indicated that DGAT1 and DGAT2 had significant relationship with backfat thickness and were taken as two candidate genes for fattening in beef cattle.