水稻叶片性状QTL分析

QTL Analysis for the Leaf Traits in Rice

以粳稻Sasanishiki和籼稻Habataki杂交衍生的回交重组自交系群体为试验材料,对叶片性状进行数量性状基因位点(QTL)进行分析。共检测到14个QTL,包括叶长、叶宽、叶面积的QTL各3个和叶厚QTL 5个,分布在第1,2,5,6,7,11和12号染色体上,贡献率在8.65%～24.45%之间。在第6号染色体上检测到1个QTL聚集区段(R566B-R1888),该区段存在着同时控制剑叶和倒二叶叶宽及叶面积的位点,贡献率均超过16.00%。相关分析表明,叶长、叶宽与叶面积之间存在极显著正相关,而与叶厚相关性没有达到显著性水平;进一步分析发现,控制叶片厚度的QTL位点与控制叶长、叶宽和叶面积的并不在相同或相近区段,可见,叶厚发育机制与叶片其他性状发育机制相关性较小。

Quantitative trait locus（QTL） controlling rice leaf characters were analyzed by using backcross recombinant inbred lines（BILs） to explore genetic mechanism of rice leaf characters.The BILs were derived from a cross between Sasanishiki（japonica） and Habataki（indica）.Fourteen QTLs were detected,including three leaf length,three leaf width,three leaf area and five leaf thickness QTL,and they distributed on the 1,2,5,6,7,11 and 12 chromosomes,explained 8.65%-24.45% phenotypic variations.One QTL cluster for the leaf width,leaf area of the flag leaf and top second leaf was revealed in region R566B-R1888 of chromosome 6,which explained more than 16.00% of phenotypic variance by a single QTL.Related analysis showed that the leaf length,width and area were very significant positive correlation,but that had no significant correlation with leaf thickness.Locus controlling leaf thickness were not in the same or similar section of chromosome that control leaf length,leaf width,leaf area.