江苏省水稻矮缩病的病原鉴定

Identification of Rice Dwarf Disease in Jiangsu

根据已发布的水稻黑条矮缩病毒(RBSDV)和南方水稻黑条矮缩病毒(SRBSDV)S7片段的序列分别设计特异性引物RB-S7-F/R和SRB-S7-F/R,采用RT-PCR、序列测定和同源性比对的方法,对2009年江苏发生的水稻矮缩病的病原进行了鉴定。结果表明,用RB-S7-F/R引物在47份检测样品中有36份可以扩增到一条1 200 bp左右的目的片段,而用SRB-S7-F/R引物未能扩增到预期条带。序列测定结果表明,扩增片段与已经发表的RBSDV中国分离物相应片段的核苷酸和氨基酸序列同源性分别为93.3%～100%和97.4%～100%,与SRBSDV相应片段的核苷酸和氨基酸同源性为77.4%～79.5%。系统进化分析得到类似结果。上述研究表明,2009年引起江苏水稻矮缩病症的病原为RBSDV,尚未发现SRBSDV的危害。

Specific primers RB-S7-F/R and SRB-S7-F/R were designed according to the published sequence of S7 segment of rice black-streaked dwarf virus（RBSDV） and southern rice black-streaked dwarf virus（SRBSDV）.Subsequently the pathogen of rice dwarf disease,which was prevalent in Jiangsu province in 2009,was identified by RT-PCR,sequencing and sequence alignment methods.The results showed that a purposed 1 200 bp fragment was cloned with RB-S7-F/R primer and wasn′t with SRB-S7-F/R in 36 of 47 detected rice samples.The sequence of this fragment shared 93.3%-100% nucleotide homology and 97.4%-100% amino acid homology with the sequence of the corresponding one in S7 of RBSDV and shared 77.4%-79.5% homology with SRBSDV.The similar result was obtained by phylogenetic relationships analysis.All above demonstrated that the pathogen of rice dwarf disease occurred in Jiangsu in 2009 was RBSDV and SRBSDV hadn′t been found in Jiangsu province.