抑制性ODN对溃疡性结肠炎患者肠黏膜单个核细胞分泌细胞因子的影响

The Effect of inhibitory ODN on cytokines expression secreted by lamina propria mononuclear cells from the patients with ulcerative colitis

目的研究抑制性ODN对溃疡性结肠炎患者肠黏膜单个核细胞分泌IL-8和TNF-α的影响。方法分离10例溃疡性结肠炎患者的肠黏膜单个核细胞,分别与含或不含CpG ODN的培养液共同培养,并用抑制性ODN或DEX加以干预。用ELISA法检测培养液中IL-8和TNF-α的水平,并用RT-PCR方法从LPMC中扩增IL-8和TNF-α基因,检测IL-8和TNF-α的mRNA表达情况。结果经检测发现抑制性ODN能明显抑制IL-8和TNF-α的分泌。结论这为研发新的治疗溃疡性结肠炎的药物提供了思路和实验依据。

Objective To study if inhibitory ODN could decrease the levels of IL-8 and TNF-αsecreted by lamina propria mononuclear cells（LPMCs） from the patients with ulcerative colitis（UC）.Methods LPMC were isolated from intestinal mucosal biopsy specimens from 10 patients with UC,and cultured with or without CpG ODN,inhibitory ODN（A151） and dexamethasone（DEX）.The levels of IL-8 and TNF-α were tested by enzyme linked immunosorbent assay（ELISA） and the expression of IL-8 and TNF-αmRNA was measured by reversal transcription-polymerase chain reaction（RT-PCR）.Results A151 resulted in down-regulation of the expression of IL-8 and TNF-α mRNA,and strikingly decreased the levels of IL-8and TNF-α,and the inhibitory effects were greater than those induced by DEX（P0.05）.Conclusion The application of inhibitory ODN may serve as a novel molecular approach for the treatment of patients with UC.