小肠结节灌注与尤斯灌流技术在猪肠道功能研究方面的应用

Application of Small Intestinal Segment Perfusion and Ussing Chamber Technique in Pig Intestinal Function

小肠灌注(Small Intestinal Segment Perfusion)技术是通过建立仔猪的原位模型,在病原菌的感染下,检测小肠的净流量和电解质浓度,从而鉴定不同益生物质在腹泻上的作用;尤斯灌流(Ussing chamber)技术通过微电极检测整个肠道细胞膜离子通道变化的电流信号,研究其药物吸收、通透性和分泌情况的变化。本文就这两种技术的发明,主要步骤和在肠道上的应用进行详细的介绍。

Small Intestinal Segment Perfusion has been developed to investigate different probiotics products effects on the net absorption of fluid and electrolytes in the small intestine of piglets under bacterial infection. The Ussing chamber provides a physiological system, where microelectrodes are used to detect current changes in intestinal cell membrane ion channels, and in studies of the absorption, permeability and secretion drugs in the intestine. In this paper, we detailed review the invention of Small Intestinal Segment Perfusion and Ussing chamber technology and its application in the pig health breeding research.