丁苯酞对不同性别hSOD1^(G93A)转基因小鼠行为学的影响

Effects of dl-3n-butyphthalide on behaviour of different sex hSOD1^(G93A) transgenic mice

目的观察丁苯酞对不同性别hSOD1^(G93A)转基因小鼠行为学的影响。方法肌萎缩侧索硬化(ALS)模型hSOD1^(G93A)转基因小鼠24只,随机分为对照组和丁苯酞组,每组雄雌各6只,分别灌胃给予玉米油10 mL·kg^(-1)·d^(-1)和丁苯酞180 mg·kg^(-1)·d^(-1),记录雄、雌小鼠的行为学表现。结果 90 d时,对照组与丁苯酞组相比均无显著差异(P>0.05)。120 d时,对照组雄性小鼠与雌性小鼠相比,旋转时间和后肢抓力均显著减小(均P<0.05),后肢步长无显著差异(P=0.857);丁苯酞组雄性小鼠与对照组雄性小鼠相比旋转时间、后肢抓力和后肢步长均显著增加(均P<0.05),2组雌性小鼠间比较,旋转时间和后肢抓力无显著差异(均P>0.05),但丁苯酞组雌性小鼠后肢步长显著增加(P<0.05)。丁苯酞组雄性小鼠比对照组雄性小鼠120 d生存期显著延长[(138.5±3.4)d vs.(119.3±3.2)d,P=0.002],雌性小鼠间无显著差异[(134.5±3.5)d vs.(132.0±2.4)d,P=0.573]。结论丁苯酞可明显提高雄性ALS模型小鼠运动协调能力和肌力,延缓疾病进展,延长生存期。

AIM To observe the effect on behavior when dl-3n-butyphthalide （NBP） exposure inhSOD1^G93A transgenic mice with different sex. METHODS Twenty four hSOD1^G93A mice for amyotrophic lateral sclerosis model were divided into control group and NBP group randomly. Every group had 6 males and 6 females. Corn oil 10 mL. kg-1. d-1 and NBP 180 mg. kg-l.d-1 were administered through oral gavages respectively. The behavior manifestations of the mice were recorded. RESULTS At 90 d, there was no significant difference between the control and NBP group （P 〉 0.05）. At 120 d, there were significant differences between male mice and female mice on rotating time and grip strength in the control group （P 〈 0.05）, but nodifference on step length （P = 0.857）. The rotating time, grip strength and step length of male mice in the NBP group were statistically increased compared with the control group （P 〈 0.05）, rotating time and grip strength of female mice had no significant difference （P 〉 0.05）, while the step length of female mice showed remarkably increase in the NBP group （P 〈 0.05） . The lifespan of males in the NBP group was remarkably lengthened compared with the control group （ 138.5 ± 3.4 vs. 119.3 ± 3.2, P = 0.002）, the lifespan of females in both groups had no obvious difference （134.5 ± 3.5 vs. 132.0 ± 2.4, P = 0.573） remarkably increase male ALS model mice ability of movement coordination and disease progress and extend the lifespan. CONCLUSION NBP can muscle strength, delay the