组蛋白去乙酰化酶1、3在炎症牙周膜中的表达

The expression of histone deacetylase 1 and 3 in inflammatory periodontal ligaments

目的:比较组蛋白去乙酰化酶1、3在炎症牙周膜和健康牙周膜中的表达差异。方法:分别收集炎症牙周膜和健康牙周膜,采用RT-PCR方法分别检测组蛋白去乙酰化酶1、3的mRNA表达,以管家基因作为内参,进行灰度值比较。结果:与健康牙周膜相比,炎症牙周膜中组蛋白去乙酰化酶1、3mRNA的表达均显著升高,差异有统计学意义(P<0.05)。结论:炎症牙周膜组织中组蛋白去乙酰化酶1、3表达升高,提示其可能参与牙周炎症反应的基因调控。

AIM： To investigate the expression of histone deacetylase 1 and histone deacetylase 3 in inflam- matory and healthy periodontal ligaments. METHODS： Inflammatory and healthy periodontal ligaments were obtained from clinical surgery patients. Histone deacetylase 1 and 3 mRNA expressions were detected by reverse transcription polymerase chain. RESULTS： The expressions of histone deacetylase 1 ans 3 mRNA were significantly higher in in- flammatory periodontal ligaments compared to those in healthy periodontal ligaments （ P 〈 0.05 ）. CONCLUSION： Histone deacetylase 1 and histone deacetylase 3 increased in inflammatory periodontal ligaments, indicating that they might be involved in the gene modulation of infammatory molecules in periodontitis.