鹰嘴豆芽素A对β淀粉样肽致阿尔茨海默病小鼠学习记忆的影响

Effect of biochanin A on learning and memory in AD model mice induced by amyloid β-protein

目的:观察鹰嘴豆芽素A对β-淀粉样肽(Aβ25-35)致阿尔茨海默病(AD)模型小鼠学习记忆的影响,并对其作用机制进行初步探讨。方法:采用一次性侧脑室注射凝聚态Aβ25-35 3μg(1.0 mmol/L)方法制备痴呆模型。造模后,对照组和模型组灌胃等体积溶剂,给药组分别给予2 mg/kg和6 mg/kg鹰嘴豆芽素A,连续灌胃14天。通过Y-迷宫和跳台实验观察鹰嘴豆芽素A对痴呆小鼠学习记忆的影响;测定小鼠血清中丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性;常规HE染色观察小鼠海马形态学改变。结果:与对照组比较,模型组小鼠学习记忆功能明显减退,海马区神经细胞离散、固缩,排列不成行;鹰嘴豆芽素A治疗组能明显增强小鼠学习记忆能力,改善小鼠海马结构,降低痴呆小鼠血清MDA含量,增强SOD活性。结论:鹰嘴豆芽素A对Aβ25-35致AD模型小鼠记忆障碍有保护作用,其作用机制可能与其抗氧化作用有关。

Objective：To investigate the effect of Biochanin A on learning and memory in AD model mice induced by amyloid β-protein.Methods：The AD model mice were established by one-time brain indoor injection condensate amyloid β-protein 3？g（10mmol/L） and then given Biochanin A in therapeutic group（2mg/kg,6mg/kg）and the same volume of solvent in control and model groups for 14 days.The learning and memory abilities of all mice were tested through Y-type maze and step-down avoidance test.Then The histomorphological features of hippocampus were observed,the content of MDA and the activity of SOD were detected.Result：Compared with the control group,the abilities of learing and memory in model group were lowered significantly.The hippocampal nerve cells became discrete,pyknotic and nonlinear.While the learning and memory abilities and structure of hippocampus in therapeutic group were improved obviously.The SOD activity was increased obviously and the MDA content was decreased signifcantly.Conclusion：Biochanin A shows protective effects on learning and memory impairment in AD mice induced by Aβ25-35.Possible mechanisms is involved in the action of antioxidation.