溃结灵对溃疡性结肠炎大鼠结肠黏膜COX-2蛋白表达和iNOS活力的影响

The effect of KuiJieling decoction on the protein expression of COX-2 and activity of iNOS in colonic mucosa of ulcerative colitis model rat

目的:观察溃结灵对溃疡性结肠炎(UC)大鼠模型结肠黏膜环氧化酶2(cyclooxygenase-2,COX-2)蛋白表达及诱导型一氧化氮合酶(inducible Nitric oxidesynthase,iNOS)活力的作用,对其抗UC作用机制进行探讨。方法:采用三硝基苯磺酸(TNBS)法复制UC大鼠模型,并进行不同剂量药物干预。采用Western blot方法检测结肠黏膜COX-2蛋白相对表达量,以及生物化学法检测结肠黏膜iNOS活力。结果:模型组COX-2蛋白表达量明显高于正常组(P﹤0.05),溃结灵高(18.3 g/kg)、中(9.2 g/kg)、低(4.6g/kg)剂量组COX-2蛋白表达量明显低于模型组(P<0.05);模型组iNOS活力显著高于正常组(P<0.01),溃结灵高(18.3 g/kg)、中剂量(9.2 g/kg)组iNOS活力明显低于模型组(P<0.05)。结论:溃结灵对TNBS法UC大鼠模型结肠黏膜COX-2蛋白表达及iN-OS活力有抑制作用,可能是其治疗UC的作用机制之一。

Objective：To observe the effect of KuiJieling decoction（KD） on the protein expression of COX-2 and activity of iNOS in colonic mucosa of Ulcerative Colitis Model Rat,and to explore its possible mechanism.Methods：Use the TNBS（trinitrobenzene sulfonic acid） clyster to establish the UC rat model and adopt different doses of the KD to intervene the development of UC.The relative protein expression of COX-2 in colonic mucosa was measured by Western blot,and the activity of iNOS in colonic mucosa was detected by biochemical kit.Results：The protein expression of COX-2 in model group was higher than that in normal group（P0.05）,the protein expression of COX-2 in three doses（18.3 g/kg、9.2 g/kg、4.6g/kg） of KD was significantly lower than that in model group（P0.05）;the activity of iNOS in model group was significantly higher than that in normal group（P0.01）,the activity of iNOS in high-dosage group（18.3 g/kg） and medium-dosage group（9.2 g/kg） of KD was significantly lower than that in model group（P﹤0.05）.Conclusion：KuiJieling decoction（KD） has inhibitory effect on protein expression of COX-2 and activity of iNOS in colonic mucosa of rats with ulcerative colitis that produced by TNBS clyster,it may be one of the mechanisms that KD reduces inflammatory reaction.