癃闭通颗粒对大鼠慢性前列腺炎模型的影响

The therapeutic effects of Longbitong Particles on the chronic prostatitis model rats

目的:研究癃闭通颗粒对慢性前列腺炎模型大鼠的治疗作用以及对正常大鼠的利尿作用。方法:采用消痔灵致雄性Wistar大鼠慢性前列腺炎模型,观察癃闭通颗粒对前列腺液内白细胞数、卵磷脂小体密度、前列腺组织匀浆中IL-8、TNF-α、IL-1β等的影响。采用给予一定水负荷方法,观察癃闭通颗粒对正常大鼠的利尿作用。采用二甲苯致小鼠耳廓肿胀模型和大鼠棉球肉芽肿模型,观察该药的抗炎作用。结果:癃闭通颗粒0.27g/kg～2.40g/kg均可降低前列腺液中白细胞数;0.80g/kg起能升高卵磷脂小体密度;0.80g/kg起能降低前列腺组织匀浆中IL-8、TNF-α、IL-1β的水平。癃闭通颗粒0.80g/kg起对正常大鼠具有利尿作用。癃闭通颗粒2.40g/kg能抑制二甲苯引起的耳廓肿胀;0.27g/kg起抑制模型大鼠的棉球肉芽肿肿胀度。提示癃闭通颗粒主要的效应剂量范围为0.80～2.40g/kg。结论:癃闭通颗粒能抑制大鼠慢性前列腺炎的炎细胞浸润,提高卵磷脂小体密度,降低前列腺组织炎性细胞因子的水平。同时具有利尿作用和抗炎作用,有利于前列腺炎的症状缓解和炎症消除。

Aim：To investigate therapeutic and diuretic effects of Longbitong particles on chronic prostatitis rats or on mormal rats.Methods：The leukocyte count,lecithin body density,IL-8、TNF-α、IL-1β are observed to investigate the therapeutic effects of Longbitong Particles on the chronic prostatitis model rats.The water-loaded rats are treated with Longbitong Particles to evaluate the diuretic effects.The anti-inflammatory effects of Longbitong particles were observed by evaluating xylene-induced ear swelling in mice and cotton-induced granuloma in rats.Result：In chronic prostatitis rats,the leukocyte count was decreased andthe lecithin body density of prostatic fluid was increased by Longbitong Particles.Meanwhile,the cytokines IL-8、TNF-α、IL-1β of prostatic homogenate can be regulated through Longbitong Particles either.The Longbitong Particles also has a diuretic effect.Longbitong particles significantly inhibited ear swelling caused by xylene in mouse and cottoninduced proliferation granuloma tissue in rats.Conclusion：The Longbitong Particles could improve inflammatory status of the chronic prostatitis model rats by regulating the expression of the related cytokines and could favour relief of symptoms through a diuretic effect.