炎症介质在重症急性胰腺炎大鼠并发脑损伤中的作用

Role of inflammatory mediators on cerebral damage caused by severe acute pancreatitis in rats

目的探讨炎症介质在大鼠重症急性胰腺炎(SAP)并发脑损伤中的作用。方法 60只雄性SD大鼠随机均分为两组:SAP模型组以0.1ml/min匀速逆行胰管注入3.5%牛磺胆酸钠0.1ml/100g;假手术组作为对照。每组又均分为3个亚组,分别于建模后3、6和12h应用放射免疫法测定血清TNF-α和IL-1β水平,采用化学比色法检测脑组织丙二醛(MDA)及超氧化物歧化酶(SOD)含量,RT-PCR法检测脑组织TNF-αmRNA和IL-1βmRNA含量,免疫组织化学法检测脑组织核因子κB(NF-κB)p65活性。结果与假手术组比较,SAP组血清TNF-α及IL-1β水平、脑组织NF-κB p65活性、脑组织MDA含量、脑组织TNF-αmRNA及IL-1βmRNA含量均随着时间的延长逐步升高,而脑组织SOD含量逐步降低(P<0.01)。结论炎症介质在SAP相关性脑损伤的发生、发展中发挥重要作用。

Objective To study the role of inflammatory mediators on cerebral damage caused by severe acute pancreatitis（SAP） in rats.Methods Sixty male SD rats were equally randomized into two groups of SAP（establishing SAP model with cerebral damage by retrograde injection of 3.5% sodium taurocholate into pancreatic duct） and C（sham operated）.The rats in two groups were equally divided into three subgroups of 3,6 and 12 hours.Serum TNF-α and IL-1β were detected by RIA.The TNF-α mRNA and IL-1β mRNA,the contents of malondialdehyde（MDA） and superoxide dismutase（SOD） and nuclear factor kappa B（NF-κB） p65 activity in brain tissues were measured with RT-PCR,chemical colorimetry and immunohistochemistry,respectively.Results Compared to group C,serum TNF-α and IL-1β,the contents of MDA,TNF-α mRNA,IL-1β mRNA and NF-κB p65 activity in brain tissues were all gradually increased,but SOD decreased,as the time prolonged（P0.01）.ConclusionInflammatory mediators play an important role in SAP with cerebral damage in rats.