非小细胞肺癌患者胸腔积液中浸润巨噬细胞趋化因子受体CX3CR1、CCR2和CCR5的表达

Expressions of chemokine receptors CX3CR1,CCR2 and CCR5 in macrophages in pleural effusion from non-small cell lung cancer patients

目的探讨非小细胞肺癌(NSCLC)胸腔积液中巨噬细胞趋化因子受体等的表达与疾病预后的相关性。方法密度梯度离心法分离恶性胸液标本获得单个核细胞;贴壁培养后,流式细胞术检测CD68、CCR2、CCR5和CX3CR1等受体的表达,分析其与肿瘤的相关性。结果巨噬细胞占整体胸液细胞的比例以及趋化因子受体的表达在不同患者胸液标本中各有不同,并与肿瘤的严重性呈现一定的相关性。结论 NSCLC胸腔积液中巨噬细胞及其趋化因子受体的表达与疾病预后有一定的相关性。检测结果可为NSCLC的诊治提供参考资料。

Objective To investigate the correlation between the expressions of chemokine receptors in macrophages in pleural effusion and the prognosis of patients with non-small cell lung cancer（NSCLC）.Methods Mononuclear cells were obtained by method of density gradient centrifugation from malignant pleural effusion,which were cultured in six-well plates for 2 hours.Adherent cells were collected and were reacted with antibodys of CD68,CX3CR1,CCR2 and CCR5 to detect the expressions of these molecules by flow cytometry.The proportion of macrophages in pleural fluid and their characteristics of profile were analyzed by statistical methods.Results The proportion of macrophages in total cells and their expression degree of chemokine receptors were varied in different samples of pleural effusion,which showed a certain degree of relevance with the severity of NSCLC.Conclusion The macrophages and their expressions of chemokine receptors are associated with the prognosis of NSCLC patients.Detecting them may be helpful in the diagnosis and treatment of NSCLC.