SSR分子标记鉴定山葡萄和河岸葡萄种间杂种

Identification of Interspecific Hybrids Derived from Vitis riparia×Vitis amurensis by SSR Marker

利用SSR分子标记技术,对山葡萄和河岸葡萄种间杂交后代的真伪性进行鉴别。从12对多态性SSR引物中筛选出能扩增出父本特异性条带的7对引物,作为杂种鉴定的标记。用这7对引物对239株山葡萄和河岸葡萄的杂交后代进行鉴定。结果表明,有161株后代具有父本的特异性条带,结合田间形态学分析,确认为真杂种。另外,后代中还出现了新的条带,表明杂交后代产生了丰富的变异。因此,SSR标记可以有效地对葡萄属种间杂交后代进行真实性鉴定,可作为葡萄种质创新的有效辅助手段。

The authenticity of 239 interspecific cross progenies from Vitis riparia 580 × Vitis amurensis 0936 was identified by SSR markers. Twelve polymorphism primers were selected randomly between two parents by SSR markers amplified,of which 7 primers with specific bands from male parent were applied to identify the authenticity of 239 progenies. The results showed that 161 of 239 progenies were all identified to be true hybrids owing to their specific bands from male parent and morphological analysis of the field. Simultaneously, there was considerable variation in the hybrids, including some with new bands. The re- sults suggest that SSR markers could be used as effective molecular markers for the authenticity,identifica- tion and genetic analysis of the progenies of grape,