多色荧光原位杂交(M-FISH)在肺癌早期诊断中的应用

Clinical application of multicolor fluorescence in situ hybridization on early diagnosis of lung cancer

目的探讨多色荧光原位杂交(M-FISH)在肺癌早期诊断中的临床意义。方法通过苏北人民医院2007年1月至2011年1月89例肺癌患者支气管镜活检组织及刷检细胞标本作为观察组。结果 89份标本中61份标本中有X染色体数目的增多,其中男性45份,女性16份。具有异常X染色体数目的细胞比例为15.1%～93.2%。26例Y染色体异常,占29.21%,其中8例Y染色体增多的标本均为男性患者,占8.99%,另18例男性患者发生Y染色体数目减少,占20.22%。结论原位荧光杂交技术对肺癌组织细胞进行检测,有助于肺癌的早期诊断,同时对于判断治疗效果、预后及有无复发等均有辅助作用。

Objective To explore the clinical significance of multicolor fluorescence in situ hybridization（M-FISH） in the early diagnosis of lung cancer.Methods The hospital from January 2007 to 2011 in January,89 cases of lung cancer patients with bronchoscopic biopsy and brush biopsy cell samples as the observation group.Results Totally 89 specimens,61 specimens,the increase in the number of X chromosomes,including 45 men and 16 women were.The proportion of cells with abnormal X-chromosome number was 15.1% to 93.2%.26 cases of Y chromosome abnormalities,accounting for 29.21%,8 cases of Y chromosome number of specimens of patients were male,accounting for 8.99%,another 18 cases of male patients with Y chromosome reduction in the number,accounting for 20.22%.Conclusions Fluorescence in situ hybridization for detection of lung carcinoma cells,contribute to the early diagnosis of lung cancer,determine treatment,prognosis,and with or without recurrence have a supporting role.