PCR-DGGE技术分析染整废水微生物群落多样性

Application of PCR-DGGE to Analysis of Microbial Community Diversity in Dyeing Wastewater

旨在揭示水解酸化-生物接触氧化工艺处理染整废水过程中的微生物多样性。取初级沉淀池,水解酸化池,生物接触氧化池和二沉池的活性污泥,通过细胞裂解直接提取基因组DNA,以细菌通用引物进行16S rRNA基因V3区域PCR扩增,将PCR产物进行变性梯度凝胶电泳,获得微生物群落的DNA特征指纹图谱,并对条带进行统计分析和切胶测序,进行了同源性分析并建立了系统发育树。研究表明,整个水处理过程中含有丰富的微生物群落,其中初级沉淀池、水解酸化池、二沉池和生物接触氧化池的污泥样品分别测出36条带、42条带、30条带和29条带。不同区段微生物群落间相似度最高达68%,最低达42.4%,说明群落间演替明显,不同工艺区段既存在共同的微生物种属也存在特异微生物种属。

In order to reveal microbial diversity of dyeing wastewater treatment which processed by hydrolytic acidification-biological contact oxidation,sludge in the primary sedimentation,hydrolytic acidification,biological contact and secondary sedimentation tank were sampled.The genomic DNA of microbial communities was extracted by cell lysis.PCR-amplified 16S rRNA which using the universal primers and denaturing gradient gel electrophoresis（DGGE） were applied to obtain the DNA fingerprint profile of the microbial communities.The bands in the gel were analyzed by statistical methods and excised for sequencing.Throughout the treatment process,microbial communities were rich.Sludge samples were measured 36,42,30 and 29 in the primary sedimentation,hydrolytic acidification,biological contact and secondary sedimentation tank.Similarity between microbial communities of different sections was up to 68.0%,the lowest of 42.4%.The profile of DGGE showed that the succession was obvious and different process sections have both different microbial species and common species.