朱砂根内源激素高效液相色谱法的测定

Studies on HPLC Method for the Endogenous Hormones of Ardisia crenata

以不同种源、不同萌发时期的朱砂根幼苗为研究对象,建立同时测定朱砂根幼苗中赤霉素(GA3)、生长素(IAA)两种内源激素的高效液相色谱(HPLC)条件。结果表明:采用Phenomenex Luna C18反相柱(5μm,250 mm×4.6 mm),甲醇和水(含0.6%乙酸)为流动相,体积比45∶55,等度洗脱,流速1.0 mL/min,柱温25℃,在波长254 nm下检测,GA3和IAA的分离效果理想,线性关系良好,回收率分别达到89.5%和87.3%。整个过程简单、易操作。比较了野生种源与栽培种源朱砂根不同苗龄期的GA3和IAA含量变化,野生种源幼苗GA3和IAA的含量较高。

A proper operation condition of the high performance liquid chromatography （HPLC） for the quantitative analysis of GA3 and IAA in Ardisia crenata was established using A. Crenata of different provenances and at different germination periods as plant material. The results showed that under the conditions of Phenomenex Luna C18 reverse phase column （5μm, 250 mm×4.6 mm）, methanol and water （with 0.6% CH3COOH） at the rate of 45/55 （v/v） as the mobile phase, isoeratic elution at the flow rate 1.0 mL/min, column temperature at 25℃, and UV wavelength at 254 nm, the separation of GA3 and IAA was ideal and the linear relationship was good. The recovery rate was 89.5% and 87.3%, respectively. Besides, the whole process was simple and easy to operate. Furthermore, GA3 and IAA contents of the wild seedlings were higher than those of the cultivated ones.