摘要
目的:建立19批不同产地来源防风的HPLC指纹图谱。方法:采用JIANGSHEN BDS C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水,梯度洗脱,检测波长为254nm,流速1.0mL.min-1。结果:对19批防风进行聚类分析和相似度计算,黑龙江拜泉等地17批药材归为推荐品,而陕西和重庆防风归为非推荐品,结果与含量测定结果一致。结论:防风的HPLC指纹图谱可用于防风的质量控制与分类评价的依据。
Objective：To develop HPLC fingerprint of 19 batches Saposhnicovia divaricata from different origins.Methods：JIANGSHEN BDS C18（250mm×4.6mm,5μm）column was used to make the determination.Methanol-water was used as mobile phase by gradient elution with the flow rate at 1.0mL·min-1.The detection wavelength was sited at 254nm.Results：The 17 batches of Saposhnicovia divaricata were classified as recommended by cluster analysis and similarity results,except ones from Shaanxi province and Chongqing,which was consistent with the content of prim-o-glucosylcimifugin and 4＇-O-D-glucosyl-5-O-methylvisamminol.Conclusion：The established HPLC fingerprint could be used as quality control and evaluation of Saposhnicovia divaricata.
出处
《辽宁中医药大学学报》
CAS
2012年第12期185-187,共3页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
防风
指纹图谱
质量控制
Saposhnicovia divaricata
fingerprint
quality control
