生防放线菌的筛选及其发酵液对小麦萌发的影响

Screening of Actinomycetes and Effect of Its Fermentation on Seed Germination of Wheat

在高氏一号培养基中加入质量浓度为50μg/mL的化学抑制剂K2Cr2O7,用抑菌圈法从陕西省洛南县土壤样品中筛选出8株放线菌,其中A 4菌株的生防效果最强,来研究A 4菌株发酵液对商麦5226和黑小麦的促生效果。结果表明,不同菌液浓度的A 4发酵液对2种小麦种子萌发和胚根的生长均具有促生作用,且对植株的生长性状影响比较显著。随着发酵液稀释倍数的增加,2种小麦的促生效果也逐渐增加,稀释到50倍时,菌液对2种小麦的诱导作用最佳。小麦培养18 h,A 4菌株发酵液稀释为50倍时,商麦5226的发芽率(95.33%)比黑小麦发芽率(90%)高5.92%,它的发芽指数(6.926)比黑小麦发芽指数(5.361)高29.19%。小麦培养第6天,商麦5226的胚根长比其对照增加57.81%,比黑小麦增加19.39%;黑小麦的胚根长比其对照增加53.26%。生防放线菌A4菌株能显著的促进商麦5226和黑小麦的生长发育。

Eight strains of actinomyces were selected to inhibit mixed bacteriums with 50 g/mL K2 Cr2 07 added into GA from soil in Luonan county of Shaanxi province. Aetinomyees A 4 had stronger biocontrol effect to the disease germs,so as to get the growth promoting to shangmai 5226 and triticale. The results showed that the different concentrations fermentation of actinomyces A 4 had growth promoting effect on Seed Germination and radicle growth of wheat, and had obvious influence on its growth traits. With increasing of dilution multiple of fermentation ,the wheat got the growth promoting effect increased gradually. When fermentation was diluted with 50 times,fermentation of actinomyces A 4 got the optimal induction to wheat. When two kinds of wheat were cultured in 18 h, actinomyees A 4 fermentation fluid was diluted with 50 times, germination of shangmai 5226 （95.33 % ） was higher than that of triticale （90%）, and germination index of shangmai 5226 （6. 926） was higher than that of triticale（5. 361 ）. Two kinds of wheat were cultured at 6 h ,radical length of shangmai 5226 was longer than ck, and it was longer than that of triticale. Radical length of triticale was longer than ck. There- fore actinomyces A 4 can improve significantly growth and development of shangmai 5226 and triticale.