小麦春化脱春化处理对POD活性的影响

Effects on POD Activity in the Leaves of Between Vernalization-Devernalization

以AK 58、百麦207、偃师4110三个小麦品种的种子为试验材料。试验共设4组,每组选用100粒种子,用75%的酒精浸泡15 min进行表面消毒。首先对3个试验组进行春化(-4℃)处理,时间分别为7、14、21 d,春化处理后放在35℃恒温培养箱中进行脱春化3 d,之后继续培养至3叶期为止;然后分别提取幼苗中过氧化物酶(POD)。并通过测定吸光值对其活性进行测定;最后通过聚丙烯酰胺电泳法显示幼苗的2种酶酶谱进行再次证实。结果表明,同一品种小麦春化、脱春化处理后小麦叶片中POD酶活性大于非处理小麦叶片的酶活性;凝胶电泳试验中,同一品种小麦叶片中的2种酶酶谱亮度强于非处理小麦叶片的酶谱,与吸光值法测定的结果相符。

The thesis selected the wheat seeds of Bainong AK 58, Bainong 207 and Yanshi 4110 as the experi- mental materials. There were four rows to be measured in the experiment. The number of the seeds in each row was about 100 ,and they should diped in the 75% alcohol for 15 minutes as artificial disinfection. First, the seeds vernalized in the experimental rows in -4 ℃ respectively in constant temperature cultivation device, the time of which was 7 d,14 d and 21 d. Then seed continued to cultivate in the temperature of 35℃ to devernal- ize, after which they grew until three-leaves phase. Second, POD enzymes was isolated from the leaves, and determined the activities of the enzymes by colorimetric detection. Third, The PAGE adopted to demonstrate the vigor of the enzymes twice. The results depicted that the enzymes, activities for one kind of wheat seeds were stronger than before. What＇ s, smore, the strips of the enzymes in the seeds after vernalization were brighter than before in the gel experiment, which is conformed to the absorption example.