浮游植物叶绿素a含量测定方法的比较及优化

Comparison and optimization of the methods for extraction and measurement of chlorophyll-a from phytoplankton

比较了丙酮萃取法和乙醇-超声波法测定水体中叶绿素a的方法,并对后者进行优化。结果显示,乙醇-超声波法的萃取效率显著地高于丙酮法的效率,两种方法的相关系数为0.9990,回归方程为Chla丙酮=1.0932Chla乙醇–11.677。优化后的乙醇-超声波法的最佳处理条件为:带样滤膜–20℃,冰冻24 h以上,加适量80℃热乙醇在80℃水浴中解冻,经超声振荡处理3 min后,室温黑暗条件下萃取6 h,再离心定容测定。

The acetone method and ethanol-ultrasonic method to extract chlorophyll a in the water were compared and the latter was optimized. The results showed that the extraction efficiency of ethanol-ultrasonic method is sig- nificantly higher than that of acetone method. The correlation coefficient of the two methods was 0.9990. The linear regression equation between the acetone and ethanol-ultrasonic method was Chla acetone = 1.0932 Chla ethanol - 11.677. After optimization, the best treatment condition for ethanol ultrasonic method was： taking sample filtration membrane at -20℃, frozen for more than 24 h, adding ethanol at 80℃ bathing with 80℃ water, oscillating with ultrasonic for 3 min, extracting for 6 h at room temperature in the dark, centrifugalizing and adding ethanol to con- stant volume for measurement.