反相离子对色谱法测定复方维生素片中3种水溶性维生素的含量

Reversed-phase ion-pair chromatography method for the determination of three water soluble vitamins in compound vitamin tablets

目的:建立反相离子对色谱法测定复方维生素片中维生素B1、维生素B2、维生素B6含量的方法。方法:以己烷磺酸钠作为离子对试剂,采用Diamonsil C18(250 mm×4.6 mm,5μm)柱,同时测定复方维生素片中维生素B1、B2和B6的含量。结果:维生素B1、B2和B6分别在6.3～14.7μg.mL-1(r=0.999 8),7.2～16.8μg.mL-1(r=0.999 5),6.3～14.7μg.mL-1(r=0.999 6)范围内线性关系良好,平均回收率分别为100.08%(RSD=0.84%,n=9),100.35%(RSD=1.26%,n=9),99.41%(RSD=1.10%,n=9)。结论:本方法快速、简便、准确,分离效果好,辅料无干扰,可用于该类制剂中维生素B1,B2和B6的含量测定及质量控制研究。

Objective： To establish an ion-pair reversed-phase chromatographic method for the determination of vitamin B1 , B2 and B6 in compound vitamin tablets. Methods： The contents of vitamin B1 , B2 and B6 were simultaneously determined by ion-pair reversed-phase high performance liquid chromatography. Results： The linear ranges of vitamin B1, B2 and B6 were 6.3 - 14.7 μg· mL^ -1 （ r = 0. 999 60 ）, 7.2 - 16.8μg· mL^ -1 （ r = 0. 999 1 ） and 6.3 - 14.7 μg· mL^ -1（r =0. 999 2） with the average recoveries of 100.08% （RSD =0.84%, n =9）, 100.35% （ RSD = 1.26% , n = 9） and 99.41% （ RSD = 1.10% , n = 9）, respectively. Conclusion ： The method is simple, rapid, accurate and reliable, and can be used for the assay and quality control of compound vitamin tablets.