海洋产毒甲藻——塔玛亚历山大藻(Alexandrium tarmarense)基因组DNA微卫星位点分离

Isolation of microsatellite sites in the toxic marine dinoflagellate Alexandrium tarmarense

采用FIASCO(Fast Isolation by AFLP of Sequences Containing repeats site)方法富集、分离海洋产毒甲藻——塔玛亚历山大藻基因组DNA微卫星位点。结果发现,测序分析的22个克隆中全部含有微卫星位点,插入片段大小为175-687 bp,微卫星DNA的重复数最低4次,最高27次,平均11次;在全部的39个微卫星位点中,其中25个属于完美重复型,13个不完美重复型,1个复合型。为塔玛亚历山大藻系统进化生物学、分子生态学研究提供了很好的标记位点。

Thirty-nine microsatellite sites in the toxic marine dinoflagellate Alexandrium tarmarense were isolated using FIASCO （ Fast Isolation by AFLP of Sequences Containing repeats site） method. The repeat number of microsatellites motif ranged from 4 to 27 with an average of 11. Among all microsatellite sites isolated, the number of microsatellite which is perfect, imperfect and compound were 25, 13 and 1, respectively. Our study offered genetic sites for systematic and evolutionary biology study and molecular ecology study of Alex- andrium tarmarense.