南京地区产超广谱β-内酰胺酶菌的流行病学调查

The Prevalence of the Genotypes of Producing Extended-spectrum Beta-lactamases Strains in Nanjing Area

目的:了解南京地区产ESBLs菌基因型的流行情况。方法:用TEM型、SHV型、CTX-M型、OXA型ESBLs的特定引物进行扩增并对扩增产物进行测序分析确定基因型。结果:30株产ESBLs大肠埃希菌和30株产ESBLs肺炎克雷伯菌未发现TEM型、SHV型、OXA型ESBLs,51株产CTX-M型ESBLs,主要为CTX-M-3、CTX-M-14。9株未扩增到ESBLs基因型。结论:南京地区产ESBLs菌基因型主要以CTX-M型为主。

Objective：To investigate the prevalence of the genotypes of producing extended-spectrum beta-lactamases （ESBLs） stains in Nanjing area. Methods： PCR was used to determine the presence of TEM,SHV,CTX-M and OXA of ESBLs by the specific primer. Then we detected the amplification products to determine their genotypes. Results： Among 80 strains of Escherichia coli and 30 strains of Klebsiella pneumoniae we didn＇t find TEM, SHV, CTX-M and OXA type ESBLs. 51 stains were detected CTX-M type ESBLs, they are mainly CTX-M-3, CTX-M-14. 9 stains were negative in PCtL Conclusion： The main genotype was CTX-M type ESBLs in the ESBLs-producing strains in Nanjing area.