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基于荧光定量PCR的兔毛、狐狸毛纤维定性检测方法 被引量:13

Qualitative analyzing method based on real-time PCR for identifying rabbit fiber and fox fiber
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摘要 通过设计针对兔、狐线粒体12SrRNA基因的特异性TaqMan探针和引物对,建立了基于DNA分析的兔毛纤维和狐狸毛纤维鉴别方法。该方法特异性好,且灵敏度达到了1%,除定性分析纤维的种类外,还可以有效鉴别微量的异纤维。 A DNA based method of identifying fiber fox fiber has been through designing sets of species-specific TaqMan probes and primers on mitochondrial 12SrRNA gene. The method features species specific and high sensitivity (1%). Besides being used for qualitative distinguish of fox fiber and rabbit fiber, it is also an effective way to identify mixed foreign fibers of micro amount.
出处 《上海纺织科技》 北大核心 2012年第11期56-58,共3页 Shanghai Textile Science & Technology
基金 国家质检总局科技项目(2011IK103)
关键词 兔毛纤维 狐狸毛纤维 DNA 物种鉴定 rabbit fiber fox fiber DNA species identification
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