菊苣酸在HepG2细胞中对SOSC3表达的调节作用

The Regulation of SOCS3 by Chicoric Acid in HepG2 Cells

探讨菊苣酸(Chicoric acid,CRA)在人肝细胞系HepG2细胞中对细胞因子信号转导抑制分子3(Suppressor of cyto-kine signaling 3,SOCS3)表达的影响,用剂量为0、5和50μg.mL-1 CRA分别处理HepG2细胞24h后,经RT-PCR、Real-time PCR检测SOCS3基因的mRNA表达情况,再以上述不同剂量CRA分别处理细胞24h以及50μg.mL-1CRA分别处理HepG2细胞0、6、12、18和24h后,用Western blot法检测SOCS3基因的表达情况。研究结果显示CRA作用于HepG2细胞后,SOCS3基因的mRNA和蛋白表达水平随着CRA剂量增加而显著下降(p<0.01),且呈现一定的剂量依赖性;随着处理时间的增加,SOCS3蛋白表达水平下降更加明显,说明在翻译水平CRA不仅呈剂量依赖性,还呈时间依赖性下调SOCS3蛋白的表达。这些结果提示CRA可抑制SOCS3蛋白在HepG2细胞中的表达。

To understand the effect of chicoric acid on the expression of suppressor of cytokine signaling 3 （SCICS3） in human heptaocellular carcinoma （HCC） cell line HepG2, the present study investigated the relationship among the mRNA level, pro- tein expression of SOCS3 and chicoric acid concentration. Firstly, HepG2 was treated with a series of concentration of chicoric acid. Secondly, the SOCS3 mRNA was assayed by semi-quantitative RT-PCR and Real-time RT-PCR 24h later, and the ex- pression of SOCS3 protein was assayed using Western Blot technique. And then, total protein expression of SOCS3 in HepG2 treated with different time（0,6,12,18,24 h）of ehieorie acid was analyzed using Western Blot. The results showed that, com- pared with control group, both of mRNA and protein expression of SOCS3 in HepG2 cells decreased significantly with the in- creasing concentration of chicoric acid in a dose-dependent manner （p^0.01）. Moreover, with the increase of manipulation time, the protein expression of SOCS3 in HepG2 cells was found to be down regulated significantly by chicoric acid in a dose- and time-dependent way. Therefore, this study shows that the chicoric acid can down regulate the expression of SOCS3 in HepG2 cells.