银灰杨和84K杨离体培养抗镉胁迫的比较

Comparative Studies on the Resistance to Cadmium Stress for Populus canescens and P. alba× P. glandulosa cv. 84K in vitro

离体条件下研究不同浓度镉胁迫处理对银灰杨和84K杨试管苗生长和抗氧化酶活性的影响,以探讨2种杨树间的抗镉性差异。结果表明:低浓度(50μmol·L-1)的镉胁迫能够促进2种杨树试管苗的生长,高浓度(200μmol·L-1)的镉胁迫则抑制植株的生长;随着镉胁迫处理浓度的增大,2种杨树试管苗的SOD酶和CAT酶活性都是先升高后下降;POD酶活性则逐渐增加。与银灰杨相比,84K杨在镉胁迫下的生长综合指标以及酶活性变化值都较低。银灰杨较84K杨有较高的抗镉污染能力。

The shoots of Populus canescens and P. alba× P. glandulosa cv. 84K in vitro were used as ma- terials to study the effects of different cadmium levels on the growth of plantlets and the activity of antioxi dant enzymes, and to reveal the difference of resistance between these two poplar varieties. The results showed that the growth of the plantlets was promoted at low Cd concentration （50 μmol·L^-1） and was inhibited at high Cd concentration （200 μmol·L^-1 ）. The SOD activity and CAT increased at first and then decreased with the increase of Cd concentration, and the POD activity gradually increased. Compared with P. canescens, under Cd stress, the comprehensive growth indices and the activity of enzymes of pop- lar 84K were lower, indicating that P. canescens had a higher capacity of antipollution of cadmium than poplar 84K.