甜菜碱缓解盐胁迫菊苣种子萌发和芽苗生长的作用

Effects of Exogenous Glycine Betaine on Seeds Germination & Bud Seedling Growth of Cichorium intybus L.Under Salt Stress

为了研究外源甜菜碱(Glycinebetaine,GB)对盐胁迫菊苣(Cichorium intybus L.)种子萌发和芽苗生长的影响,以不同浓度(0～280mmol/L)的NaCl结合外源GB(0～10.0mmol/L)处理菊苣种子并进行发芽试验,测定了种子的发芽势、发芽率、发芽指数和活力指数等指标。结果表明:盐溶液抑制了种子的萌发。其中,280mmol/LNaCl能完全抑制种子的萌发;外源GB可以不同程度的缓解盐胁迫的抑制作用,且具有浓度依赖效应,其中2.5mmol/LGB的缓解作用最好,能显著提高210mmol/LNaCl胁迫下菊苣种子的发芽势、发芽率和发芽指数(P<0.05);外源GB对盐胁迫下芽苗的鲜重和干重无明显的缓解作用,外源GB能缓解盐胁迫对菊苣种子萌发的抑制作用。

The aim was to investigate the protective effect of exogenous glycine betaine（GB） solution（010.0 mmol/L） on seeds germination and bud seedling growth of Cichorium intybus L.under salt stress.Seeds germination test were carried out by treated seeds with the combination of different concentrations of NaCl（0-280 mmol/L） and 0-10.0 mmol/L GB.The results showed that,germination of seeds was inhibited by salt solution.280 mmol/L NaCl completely inhibited the seed germination.Exogenous GB could markedly alleviate salt stress with an obvious dose-dependent effect.Among them,2.5 mmol/L GB was the optimium dose,which could significantly promoted germination percentage,germination potential and germination index under the solution of 210 mmol/L NaCl significantly（P0.05）.However,exogenous GB could not alleviate the inhibitory action of salt stress on bud seedling growth.Exogenous GB could alleviate the inhibition effects of salt stress on seed germination of Cichorium intybus L..