乙酰甲喹在鲤鱼肌肉组织中的残留消除规律研究

Studies on the Elimination of Mequindox in Cyprinus carpio

为研究乙酰甲喹(Mequindox)在鲤鱼体内的残留消除规律,为水产品质量安全提供基础数据。在水温(15±1)℃条件下,给鲤鱼单次口灌乙酰甲喹(10mg/kg)后,定时取样,采用HPLC法测定肌肉中药物浓度。结果表明:通过DAS2.0药代动力学软件非房室模型统计矩原理计算各项参数,主要动力学参数分别为:消除半衰期t1/2为0.17h,达峰时间Tmax为0.50h;最高药物浓度Cmax为761.75μg/L;总体消除率CLz/F为42.59(L.h/kg),1.5h后几乎检测不到原药;药时曲线出现双峰现象,表示可能存在肝肠循环或胃肠循环。乙酰甲喹在鲤鱼体内吸收良好、代谢快、体内残留少。

In this article,elimination of mequindox residue in Cyprinus carpio muscle tissue was studied so as to provide basic data for the quality and security of aquatic products.The experiment was conducted at 15℃.After oral treatment at a single dose of 10 mg/kg,the fish was sampled at certain time and the concentrations of mequindox in muscle were determined by high performance liquid chromatography（HPLC）.The results showed that the concentration-time data of mequindox was analyzed with the pharmacokinetics software DAS 2.0.Rosenblueth method of non-compartment model was used to calculate the parameters of mequindox.The main pharmacokinetic parameters were as follows：the terminal elimination half-life（t 1/2） was 0.17 h.The times of peak concentration（T max） was 0.50 h.The maximum concentration（C max） was 761.75 μg/L.The body clearance of the drug was 42.59（L.h/kg）.The mequindox concentration was below detection limits after 1.5 h.The drug concentration versus time curve showed an innormal double-peak phenomenon.The double-peak of concentration meant that there might be hepatoenteral circulation or stomach intestine circulation during the metabolism process.The results showed that the pharmacokinetic characteristics of the mequindox in Cyprinus carpio were as follows：fast absorption,wide distribution,rapid elimination and low drug residues.