亚硝酸钠和精氨酸对白桦悬浮细胞中NO和三萜合成的影响

The Effect of Sodium Nitrite and Arginine on the Biosynthesis of Nitric Oxide and Triterpenoid in Birch(Betula platyphylla Suk) Suspension Cells

旨在分析亚硝酸钠和精氨酸处理12h后白桦悬浮细胞中一氧化氮(NO)和三萜含量的变化。在白桦悬浮细胞的生长末期添加硝酸还原酶(NR)和一氧化氮合成酶(NOS)的底物亚硝酸钠和精氨酸,采用比色法和荧光显微镜方法分析白桦悬浮体系中三萜含量和NO含量的变化。500μg/L和100μg/L的亚硝酸钠和精氨酸均促进了白桦悬浮细胞中NO和三萜的合成,其中500μg/L的亚硝酸钠和精氨酸促进作用最强。500μg/L亚硝酸钠处理下白桦细胞中的NO荧光强度和三萜含量均高于500μg/L精氨酸处理,其中三萜含量增加了1.5倍。将NR和NOS的抑制剂NaN3和L-NAME分别添加到亚硝酸钠和精氨酸处理的白桦悬浮体系中,发现亚硝酸钠和精氨酸对NO和三萜合成的促进作用被抑制了。NR和NOS来源途径的NO参与了白桦三萜的合成,可能NR来源途径的贡献大于NOS途径。

The aim was to analyze the relation of nitric oxide （NO） and triterpenoid production. Sodium nitrite and arginine were added in the eight-day-old birch suspension cell culture, the change of triterpene content and NO content were analyzed by chemical colorimetry and fluorescence microscopy. NO and triterpenoid content were increased by 500 μg/L and 100 μg/L sodium nitrite and arginine, and the higher increasing was induced by 500 μg/L sodium nitrite and arginine. The content of NO and triterpenoid in birch cells induced by 500 μg/L sodium nitrite was higher than that of 500 μg/L arginine, the increasing rate of triterpenoid was 1.5 times. Inhibitor of NaN3 and L-NAME decreased the effect of sodium nitrite and arginine on the biosynthesis of NO and triterpenoid, the content of NO and triterpenoid were near to the level of control. These results preliminary indicated that NR and NOS were involved in triterpenoids biosynthesis in birch suspension culture, and NR might be play an important role in triterpenoids biosynthesis.