桃芽变品种的RAPD分析

RAPD Analysis on Peach Buds Change of Varieties

为了从遗传学基础上对9个桃品种进行分类及亲缘关系研究,利用随机扩增多态性DNA(RAPD)技术对其基因组DNA进行标记,并应用SPSS11.0软件进行聚类分析。聚类分析结果表明:不同桃品种在欧氏距离平方系数阈值为2.0～6.0时,聚为2类,第1类由‘满园红’、‘中油4号’、‘中油9号’和07-9-6组成,均为油桃;第2类由97-6-83、NJC83、‘皖83’、NJC19和07-9-19组成,均为黄桃。在欧氏距离平方系数阈值为1.0～2.0时,所有品种聚为4类,NJC83和其营养系变异97-6-83、‘皖83’聚为一类,NJC19和其变异07-9-19聚为一类,‘中油4号’和其营养系变异‘满园红’聚为一类,‘中油9号’和其变异09-6-9聚为一类。RAPD分析认为:母本与营养系变异间的遗传距离短,具有很近的亲缘关系,其在DNA水平上产生了一定量的变异性条带,出现了较大范围的变异。

In order to classify the nine peach varieties and research the genetic relationship from the genetic basis,random amplified polymorphic DNA（RAPD） technology was used to mark the genomic DNA and the cluster analysis was performed with SPSS 11.0.The results of cluster analysis showed that when the value of Squared Euclidean distance coefficient was from 2.0 to 6.0,the different peach varieties were divided into two groups.The first group was made up of ＇ Manyuanhong ＇,＇ Zhongyoutao 4 ＇,＇ Zhongyoutao 9 ＇ and 07-9-6.They were all considered as nectarine.The second group was made up of 97-6-83,NJC83,＇ Wan83 ＇,NJC19 and 07-9-19.They were all considered as yellow peach.All the varieties were divided into four groups when the value of Squared Euclidean distance coefficient was from 1.0 to 2.0.NJC83 and its clone mutants（97-6-83 and ＇ Wan83 ＇） were in the same group.NJC19 and its variation（07-9-19） got together.＇ Zhongyoutao 4 ＇ and its clone mutant（＇ Manyuanhong ＇） gathered together.＇ Zhongyoutao 9 ＇ and its variation（09-6-9） clustered together.The RAPD analysis indicated that the genetic distance between the female parent and its mutant was short.They had very close relationship.They have produced a certain amount of specificity strips and also emerged a wide range of variation.