西太公鱼微卫星的筛选与分析

Isolation and characterization microsatellite markers from Hypomesus nipponensis

利用磁珠富集法从西太公鱼(Hypomesus nipponensis)基因组中筛选到14对多态性微卫星引物,并对来自黑龙江镜泊湖的西太公鱼24个样本进行检测:扩增的等位基因数在3～25个之间,平均为8.4个;观察杂合度(HO)和期望杂合度(HE)分别为0.266 7～1.000 0和0.282 5～0.9322;多态信息含量(PIC)为0.239 2～0.910 6;位点HN-075和位点HN-250显著偏离哈迪-温伯格平衡(P<0.05),这些多态性微卫星位点可用于公鱼属鱼类种群遗传结构及其相关研究。

Fourteen polymorphic microsatellite loci were isolated from the Japanese smelt（Hypomesus nipponensis）.These microsatellite markers were tested in a sample of 24 individuals.The number of alleles ranged from 3 to 25,with an average of 8.4 alleles per locus.The observed and expected heterozygosity of these loci varied from 0.266 7 to 1.000 0 and 0.282 5 to 0.932 2,respectively.Locus HN-075 and HN-250 deviated significantly from Hardy-Weinberg equilibrium（P0.05）.No genotypic disequilibrium among loci was found（P0.05）.These microsatellite markers could be useful in studing population structure of the Japanese smelt,or introduction routes of this fish to China.