摘要
[ Objective ] This study aimed to explore the ways of using SSR technology to construct the fingerprinting maps of the loquat. [ Method ] Fingerprinting patterns of the SSR markers for 33 loquat varieties in national loquat germplasm resource garden were constructed by specifically amplifying palymorphism from 7 pairs of primers screened from 51 pairs of primers of the relatives Mains and Pyrus. [ Result ] The results showed that the uses of 7 primers, namely, Hi01d05F, Hil2iO4F, CH02B10F, Hi03a03, Hi03e04, Hi04a05 and Hi02c07 could distinguish 33 loquat germplasms from one another completely. [ Conclusion] This study provides an essential criterion for classification and identification of the loquat resources.
[ Objective ] This study aimed to explore the ways of using SSR technology to construct the fingerprinting maps of the loquat. [ Method ] Fingerprinting patterns of the SSR markers for 33 loquat varieties in national loquat germplasm resource garden were constructed by specifically amplifying palymorphism from 7 pairs of primers screened from 51 pairs of primers of the relatives Mains and Pyrus. [ Result ] The results showed that the uses of 7 primers, namely, Hi01d05F, Hil2iO4F, CH02B10F, Hi03a03, Hi03e04, Hi04a05 and Hi02c07 could distinguish 33 loquat germplasms from one another completely. [ Conclusion] This study provides an essential criterion for classification and identification of the loquat resources.
基金
Supported by the High-technology Program of Jiangsu Province(BG2007312)
