桃金娘对颈动脉血栓形成的影响及其机制的实验研究

Effect of myrtle on thrombosis and experimental study on its mechanism

目的探讨桃金娘对血栓形成的影响及其机制。方法经大鼠颈静注射桃金娘5、10 mg/kg,用血栓生成仪观察大鼠颈动脉血栓形成情况;分别采用全血与桃金娘及生理盐水孵育5 min,用全血血小板聚集仪观察全血血小板聚集情况;用ELISA法检测血小板环磷酸腺苷(cAMP)和环磷酸鸟苷(cGMP)的含量;用硝酸还原酶法观察血小板释放NO情况。结果桃金娘5 mg/kg和10 mg/kg能够促进大鼠颈总动脉血栓形成,堵塞率分别为(87.46±10.31)%和(95.26±12.42)%。200、400和800μg/mL的桃金娘均能够诱导全血血小板聚集,其最大聚集率分别为(0.75±0.50)、(3.75±2.36)和(7.75±0.96)Ω。200、400和800μg/mL的桃金娘均能减少血小板cAMP、cGMP和NO的生成,含量分别为[(22±5.05)、(18.71±5.11)、(15.62±6.19)pmol/mL]、[(6.54±1.86)、(5.34±2.36)、(5.03±1.67)nmol/mL]和[(120.89±26.34)、(104.28±21.27)、(86.61±16.98)μmol/mL]。结论桃金娘能促进血栓形成和血小板聚集,可能机制为下调血小板cAMP、cGMP和NO水平。

Objective To investigate the effect of myrtle on thrombosis and its mechanism. Methods SD rats were given myrtle （5,10 mg/kg） transjugular injection, and the thrombosis in the carotid artery of rat was observed by a throm- bosis inducer; Whole blood was incubated with myrtle or saline for 5 mins, and then platelet aggregation was analysed by whole blood aggergometers; Concentration of cyclic adenosine monophosphate（cAMP） and cyclic guanosine monophosphate （cGMP） was measured by ELISA; NO concentration was measured by nitrate reductase-dependent assay. Results Myrtle of 10 mg/kg and 20 mg/kg increased thrombosis in the carotid artery of rat with the block rate of（87.46 ± 10.31 ）% and （95.26± 12.42 ）%, respectively. Myrtle of 200,400 and 800 μg/mL increased platelet aggregation with the maximum ag- glutination of（0.75 ± 0.50 ）, （ 3.75 ± 2.36 ） and （ 7.75 ± 0.96 ） Ω, respectively. Myrtle 200, 400 and 800 μg/mL de- creased the production of cAMP, cGMP and NO in platelets with the concentration of [ （22± 5.05 ）, （18.71 ± 5.11 ）, （15.62±6.19） pmol/mL], [（6.54±1.86）, （5.34±2.36）, （5.03±1.67） nmol/mL] and [（120.89±26.34）, （ 104.28 ± 21.27）, （86.61± 16.98 ） μmol/mL ], respectively. Conehtsion Myrtle can increase thrombosis and platelet aggregation, the mechanism may due to the down regulation of cAMP, cGMP and NO in platelets.