锌对不同发育时期子代果蝇基因组DNA甲基化的影响

Effect of zinc on DNA methylation diversity at different developmental stages of filial generation in Drosophila

为了初步探讨微量元素锌对不同发育阶段子代果蝇基因组DNA甲基化的影响及可能机制,以黑腹果蝇(Drosophila melanogaster)Canton-S品系为实验材料,应用改良的AFLP分子标记技术分析了果蝇基因组DNA甲基化多态性,以及不同浓度(2.5%、5%、10%)锌处理亲代果蝇后,不同发育时期的子代果蝇基因组DNA甲基化的改变。结果显示:胚胎、三龄幼虫、蛹期、雌及雄蝇DNA甲基化多态条带比率均存在显著性差异;亲本果蝇经锌处理后,其5-8h胚胎DNA甲基化多态条带比率在3个实验组中均显著高于对照组;锌浓度为2.5%时,子代果蝇DNA甲基化所受影响最大,在5-8h胚胎、12-15h胚胎、18-21h胚胎及雌蝇中,DNA甲基化多态条带比率均显著高于对照组。结果表明胚胎期DNA甲基化多样性最高,同时适当浓度锌处理,可提高子代果蝇基因组DNA甲基化多态性。

To examine the features of DNA methylation diversity, we used a zinc treated Canton-S strain of Drosophila melanogaster as a model organism in this experiment. Our results showed significant differences in DNA methylation polymorphisms among embryos, third instar larvae, pupae, and female and male individuals. After zinc treatment （at a concentration of 2.5%, 5%, and 10%） to the parental generation, the percentage of DNA methylation polymorphic bands of offspring （5-8 h embryo） in the experimental groups F1-1, F1-2, F1-3 were significantly higher than the control group. The experimental group F1-2 （zinc concentration 2.5%） in 5-8 h embryo was affected most by zinc treatment of the parental generation, and DNA methylation polymorphic band ratios in 12-15 h embryo, 18-21 h embryo and female D. melanogaster were significantly higher than the control groups. Our results indicate that the embryo stage contains the highest DNA methylation diversity, and appropriate concentration of zinc material can increase DNA methylation diversity olD. melanogaster offspring.