维生素E对二甲基甲酰胺致小鼠急性肝损伤的拮抗作用

Antagonistic effect of vitamin E on acute liver injury induced by N,N-dimethylformamide in mice

目的探讨维生素E(Vitamin E,VE)对二甲基甲酰胺(N,N-dimethylformamide,DMF)致急性肝损伤的拮抗作用。方法将50只健康SPF级雄性昆明小鼠随机分为5组,分别为正常对照组、VE(10 mg/kg)对照组、DMF中毒模型组及低(5 mg/kg)、高(10 mg/kg)剂量VE保护组,每组10只。VE对照组和低、高剂量VE保护组采用灌胃方式染毒VE溶液,正常对照组和DMF中毒模型组分别染毒等体积玉米油,每天1次,连续5 d;最后1次染毒VE后0.5 h,DMF中毒模型组及各剂量VE保护组一次性灌胃染毒2 g/kg的DMF溶液,同时,正常对照组和VE对照组给等体积蒸馏水。检测小鼠血清丙氨酸氨基转氨酶(ALT)、天冬氨酸氨基转氨酶(AST)和黄嘌呤氧化酶(XOD)活力,肝脏匀浆中谷胱甘肽(GSH)和脂质过氧化产物丙二醛(MDA)的含量。结果与DMF中毒模型组比较,VE保护组小鼠血清ALT、AST、XOD活力显著降低(P<0.05),肝脏GSH含量明显升高(P<0.05),MDA含量显著下降(P<0.05)。结论 VE对DMF引起的小鼠肝损伤具有拮抗作用。

Abstract：Objective To study the antagonistic effect of vitamin E （VE） on acute liver injury induced by N, N- dimcthylformamide （DMF）. Methods A total of 30 healthy SPF male KM mice were randomly divided into five groups：normal control group, VE （10 mg/kg） control group, DMF poisoning model group, low dose （5 mg/kg） and high dose （10 mg/kg） VE- protected groups. VE control group, low dose and high dose VE-proteeted groups were treated with VE solution through garage. Simultaneously, normal control group and DMF poisoning model group were given corn oil. The garage volume was 2.5 ml/kg, once a day, tot five consecutive days. 0.5 h after the last VE-gavage, DMF poisoning model group and each VE-protccted group were given 2 g/kg DMF one time. Simultaneously, normal control group and VE control group were given distilled water one time. The gavage volume was 5 ml/kg. 24 h after DMF poisoning, the activities of serum ALT, AST, XOD and the contents of hepar CSH, MDA were detected. Results Compared with the DMF poisoning model group, the activities of serum ALT, AST, XOD in VE-proteeted groups decreased significantly （P〈0.05）, the content of hepar GSH increased significantly （P〈0.05）, and the content of hepar MDA decreased significantly （P〈O.O53. Coilclusion VE may have effective antagonism on the liver injury induced by DMF.